静脉输注高氧液对急性一氧化碳中毒大鼠生化指标的影响  

The effects of hyperoxygen solution injected in vein on biochemical indexes of rats with acute carbon monoxide poisoning

作　　者：徐浩 孟祥忠 高巍 宋博 徐礼鲜 张惠 

机构地区：[1]第四军医大学口腔医院麻醉科,西安710032

出　　处：《国际麻醉学与复苏杂志》2011年第5期537-540,共4页International Journal of Anesthesiology and Resuscitation

基　　金：基金项目：国家科技重大专项项目（2008ZXJ09004-010）;全军十一五科技攻关项目（2006G093）

摘　　要：目的本研究旨在探讨静脉输注高氧液（hyperoxygen solution，HOS）对急性一氧化碳（CO）中毒大鼠生化指标的影响。方法采用随机数字表法将18只SD大鼠随机分为3组（每组6只）：正常组（N组）、中毒组（C组）和中毒治疗组（H15组）。在中毒后1h，C组输注平衡盐溶液15ml／kg，H15组输注HOS15ml／kg。大鼠经腹腔注入CO 120ml／kg建立中毒模型，N组给予同等剂量的空气。各组大鼠分别在输注不同剂量的液体后即刻抽取动脉血0．5ml进行血气指标检测，染毒后1d抽取静脉血3ml进行脑、心、肝、肾敏感生化指标的测定。结果CO 120ml／kg腹腔注射1．5h能引起严重的低氧血症动脉血氧分压（PaO2）由（96．6±2．4）mmHg（1mmHg=0．133kPa）降低到（43．O±4．1）mmHg。中毒后1d能够引起神经元特异性烯纯化酶（neuron-specific enolase，NSE）、S-10013蛋白、肌酸激酶（creatine kinase，CK）、肌酸激酶同工酶（ereatine kinaseMB，CK-MB）、乳酸脱氢酶（lactate dehydrogenase，LDH）、a-羟丁酸脱氢酶（a-hydroxybutyrate dehydrogenase，a-HBDH）、总胆红素（total bilirubin，TBIL）、谷草转氨酶（aspartate aminotransferase，AST）、谷丙转氨酶（alanine aminotransferase，ALT）、谷氨酰转移酶（glutamyl transferase，GGT）、尿素氮（blood urea nitrogen，BUN）和肌酐（creatinine，Cr）的明显升高，CO中毒组与正常对照组相比差异有统计学意义（P〈0．01）。静脉输注HOS后，血浆溶解氧的含量显著升高，PaO2由（43．0±4．1）mmHg上升到（77．1±2．5）mmHg。各种生化指标也均有不同程度的降低，虽然明显高于正常对照组，但与CO中毒组相比各种指标的升高均有所降低（P〈0．01）。结论经静脉输注HOS15ml／kg能明显降低急性CO中毒大鼠血浆各种生化指标的含量，对急性CO中毒引起的重要脏器损害具有很好的保护作用。Objective To investigate the effects of hyperoxygen solution （HOS） injected in vein on biochemical indexes of rats with acute carbon monoxide poisoning. Methods Eighteen SD rats were randomly divided into 3 groups, Normal group （N group）, carbon monoxide poisoning group （C group） and HOS treatment groups（H15 group）. Each contained 6 rats. After exposure of CO poisoning 1 h, C group accepted with balanced sah solution 15 ml/kg and H15 group with HOS 15 ml/kg. CO poisoning model were established by intraperitonea] injection CO with 120 ml/kg. C group and the treatment group were such poisoning rats, while N group were given the same dose of air. At the end of treatments, all the rats were collected 0.5 ml of arterial blood for blood gas measurement and 3 ml of venous blood for the determination of sensitive biochemical indexes in brain, heart, liver, and kidney. Results CO 120 ml/kg with intraperitoneal injection can cause severe hypoxemia after 1.5 h, PaO2 decreased from the （96.62 ± 2.4） mm Hg （1 mm Hg=0.133 kPa） to （43.04±4.13） mm Hg. After CO poisoning for 1 d, C group had test indicators increased significantly including neuron-specific enolase （NSE）, troponin （cTnT）, creatine kinase （CK）, ereatine kinase MB （CK-MB）, lactate dehydrogenase （LDH）, a-hydroxybutyrate dehydrogenase （a-HBDH）, total bilirubin （TBIL）, aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, glutamyl transferase （GGT）, blood urea nitrogen （BUN） and creatinine （Cr） （P〈0.01）. When intravenous infused with different doses of HOS, Dissolved oxygen in plasma was significantly increased, PaO2 increased from the （43.0± 4.1） mm Hg to （77.1±2.5） mm Hg. All the enzymes were significantly lower than these in C group, although still were significantly higher than these in N group （P〈0.01）. Conclusion Intravenous infusion with HOS 15 ml/kg can reduce significantly the biochemical indexes changes caused by acute CO poisoning in rat, and preve

关 键 词：一氧化碳中毒 高氧液 生化指标 

分 类 号：R595.1[医药卫生—内科学]