绿豆幼苗对Cr^(3+)胁迫响应的研究  

Research on the Response of Green Bean Seedlings to the Stress of Cr^(3+)

作　　者：庄媛[1] 王小平[1] 

机构地区：[1]南京晓庄学院生命化工与环境工程学院,江苏南京211171

出　　处：《安徽农业科学》2011年第26期15867-15868,共2页Journal of Anhui Agricultural Sciences

基　　金：南京晓庄学院自然科学基金项目(2010KYYB14)

摘　　要：[目的]研究绿豆幼苗在生长过程中受Cr3+胁迫后的生长生理等情况。[方法]采用沙培法进行探究试验,分析Cr3+胁迫环境下绿豆幼苗的响应。[结果]浓度<90 mg/L的Cr3+能增加绿豆幼苗根的活力,而随着Cr3+浓度的增加,根的活力则下降,根老化加快,如当Cr3+浓度为150 mg/L时,其活力为对照的56%。幼苗体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等抗氧化酶对Cr3+浓度反应也不同,当Cr3+浓度在10~90 mg/L时,其活性呈现上升趋势并高于对照,之后则呈下降趋势并低于对照;当Cr3+浓度为150 mg/L时,SOD、CAT活性分别为对照的51%和53%;脯氨酸和丙二醛(MDA)的含量随Cr3+浓度增加而增加。[结论]低浓度(≤50 mg/L)的Cr3+对绿豆幼苗生长影响不大,并能提高植株的抗逆性,而高浓度Cr3+则抑制其生长。[Objective] The growth and physiological character of green bean seedling,which growth process was under the Cr3＋ stress,was researched.[Method] The response of bean seedling that was cultured in sands to the environmental stress of Cr3＋ was analyzed.[Result] The treatment of Cr3＋ with the content of 90 mg/L could increase the root vitality of bean seedling,and with the concentration-increasing of Cr3＋,its root vigor decreased and its root aging sped up,for example,when the content of Cr3＋ was 150 mg/L,its root activity was 56% of the CK＇s.The response of bean seedling to the treatment of Cr3＋ in the antioxidant enzyme such as superoxide dismutase（SOD） and catalase（CAT） and others was different and when the concentration of Cr3＋ was in the range of 10-90 mg/L,its root activity rose,which was higher than that of CK,then,it was in downward trend,which was lower than that of CK.When the concentration of Cr3＋ was 150 mg/L,the activity of SOD and CAT was 51% and 53% of CK,respectively.The content of proline and MDA was raised with the concentration-increasing of Cr3＋.[Conclusion] The treatment of low concentration of Cr3＋（≤ 50 mg/L） had little effect on the growth of bean seedling and could improve its plant resistance but the treatment of high concentration of Cr3＋ would inhibit the bean seedling growth.

关 键 词：CR3+ 胁迫 绿豆 幼苗 

分 类 号：S522[农业科学—作物学]