吴茱萸中7种活性成分的含量测定  

Determination of the Contents of Seven Active Components in FRUCTUS EVODIAE

作　　者：曾金祥[1] 朱继孝[1] 黄碧涛[2] 王劭华[1] 杨雅琴[1] 王晓云[1] 

机构地区：[1]江西中医学院江西省中药种质资源工程技术研究中心,江西南昌330004 [2]南昌市洪都中医院,江西南昌330008

出　　处：《安徽农业科学》2011年第26期15988-15990,共3页Journal of Anhui Agricultural Sciences

基　　金：江西中医学院重点学科青年教师培养计划资助项目(吴茱萸质量控制技术研究;编号:2010023);国家"十二五"科技支撑计划资助项目(龙脑樟等6种大宗药材规范化种植基地及其SOP优化升级研究);编号:2011BAI04B04

摘　　要：[目的]采用高效液相色谱及紫外检测法建立同时测定吴茱萸药材中辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱7种活性成分含量的方法。[方法]采用浓度为50%的甲醇-水溶液超声制备样品。以Hypersil C18(5μm,250 mm×4.6 mm)为色谱柱,以乙腈-乙酸(V/V,1%)水溶液为流动相进行梯度洗脱。在254 nm波长下检测芦丁、金丝桃苷和槲皮素的含量;在270 nm波长下检测辛弗林和吴茱萸碱的含量;在340 nm波长下检测吴茱萸次碱和绿原酸的含量。[结果]辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱各成分分离效果良好;其平均回收率均接近100%,RSD均小于3.1%。[结论]该方法适用于同时测定吴茱萸药材中辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱的含量,为吴茱萸药材的质量评价提供了更好的技术手段。[Objective] To establish a HPLC/DAD（photodiode-array detection） method for simultaneous determination of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine,and rutaecarpine in FRUCTUS EVODIAE.[Method] The samples were prepared with 50% methanol aqueous solution by sonication.The chromatographic separation was performed on a C18 column（250×4.6 mm,5 μm） and the acetonitrile-1% acetic acid was mobile phase with gradient dilution.The flow rate was 1.0 ml/min,and the detection wavelength was 254 nm for rutin,hyperoside and quercetin,270 nm for synephrine and rutaecarpine,and 340 nm for chlorogenic acid and evodiamine,respectively.[Result] The extraction effect of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine,rutaecarpine and evodine was good.Their mean recoveries approached 100%,and RSDs were below 3.1%.[Conclusion] The method was suitable for the simultaneous determination of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine and rutaecarpine,and provided better technical support for the quality assessment of FRUCTUS EVODIAE.

关 键 词：吴茱萸(EVODIAE FRUCTUS) HPLC/DAD法 活性成分 含量测定 

分 类 号：S567.239[农业科学—中草药栽培]