罗布麻叶总黄酮提取物对抑郁大鼠脑内单胺类神经递质含量的影响  

Influence of Flavonoids Extracted from Apocynum venetum L. Leaves on the Content of Monoamine Neurotransmitters in Rat Brain Tissue

作　　者：郑梅竹[1] 时东方[1] 刘春明[1] 王秋悦[2] 张语迟[1] 吴桂梅[1] 

机构地区：[1]长春师范学院中心实验室,吉林长春130032 [2]河北科技师范学院动物科学系,河北秦皇岛066600

出　　处：《安徽农业科学》2011年第26期15995-15997,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(30970299);吉林省科技厅重点项目(20090936);吉林省教育厅项目([2009]D206)

摘　　要：[目的]探讨罗布麻(Apocynum venetum L.)叶总黄酮提物抗抑郁作用的机理。[方法]采用大鼠慢性应激抑郁模型,利用液相色谱-质谱串联法测定大鼠脑内单胺类神经递质[去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)]的含量。[结果]慢性应激抑郁组动物组织内DA、5-HT和NE含量与正常组相比均极显著降低(P<0.01)。罗布麻总叶黄酮提取物处理组大鼠脑中DA和NE含量与慢性应激抑郁组相比显著或极显著上升,且随着剂量的增大,这种作用更加明显;70.0 mg/kg罗布麻叶黄酮提取物处理与盐酸氟西汀(3.5mg/kg)效果一致;但罗布麻叶总黄酮提取物处理组大鼠脑中5-HT含量与慢性应激抑郁组相比没有明显变化,阳性对照药氟西汀显著增加了抑郁大鼠脑组织中DA、NE和5-HT含量。[结论]该研究证实了抑郁症发病的神经递质假说,同时发现罗布麻叶可以逆转抑郁大鼠脑内单胺类物质NE、DA含量的降低,这也可能是其抗抑郁作用的机理之一。[Objective] To study the antidepressant effect and mechanism of flavonoids extracted from Apocynum venetum leaves.[Method] The LC-MS/MS method was applied to investigate the contents of monoamine neurotransmitters [norepinephrine（NE）,5-hydroxytryptamine（5-HT） and dopamine（DA）] in rats brain tissue by chronic stress of depression model.[Result] The concentration of NE,DA and 5-HT in chronic stress depression rats＇ brain decreased compared with the control group,while the contents of NE and DA in AV-extract group were higher than depression group,and the effect was more apparently with the increasing dose of Apocynum venetum L..When treated with AV-extract（70.0 mg/kg）,the results were similar to fluoxetine（3.5 mg/kg） group.However,5-HT content of the AV-extract group was close to the depression group,but the increased dose of fluoxetine improved the content of DA,NE and 5-HT in the rat brain tissue of the depression group.[Conclusion] The study proved the neurotransmitter hypothesis of depression,and found out that AV-extract could reverse the decrease of NE and DA content in rat brain tissue,which might be the antidepressant mechanism of of AV-extract.

关 键 词：罗布麻 抑郁 单胺递质 

分 类 号：S567[农业科学—中草药栽培]