HPLC测定金银花及金芪降糖片中6种成分的含量  

Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC

作　　者：于静[1] 邓雁如[1] 陈奇[1] 宋丽丽[1] 高樱[1] 郭利平[1] 

机构地区：[1]天津中医药大学天津市中药化学与分析重点实验室,天津300193

出　　处：《中国实验方剂学杂志》2011年第19期57-60,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展规划(973)项目(2009CB523003)

摘　　要：目的:建立同时测定金银花及金芪降糖片中6种成分的反相高效液相色潽法。方法:色谱柱为依利特ODS C18(4.6 mm×250 mm,5μm);乙腈-0.1%磷酸溶液为流动相,梯度洗脱;流速1 mL.min-1;检测波长355 nm;柱温30℃。结果:6种成分在上述条件下分离良好。绿原酸、咖啡酸、芦丁、金丝桃苷、木犀草苷和4,5-O-双咖啡酰奎宁酸分别在3.24～64.80,0.24～4.79,1.22～24.40,0.09～0.44,0.68～13.55,1.19～23.87 mg.L-1线性良好,平均回收率均大于97%(RSD<3%,n=9)。结论:本方法操作简单、结果准确、回收率高、重复性好,可用于金芪降糖片及其相关制剂的质量控制。Objective：To establish a high performance liquid chromatography method of simultaneously determining 6 constituents in Flos Lonicerae and Jinqi Jiangtang tablets.Method： The separation was carried out on an ODS C18 column（4.6 mm×250 mm,5 μm） with acetonitrile-0.1% H3PO4 as mobile phase by gradient elution.The flow rate was 1 mL·min-1 and the detection wavelength was at 355 nm.The column temperature was at 30 ℃.Result： The six ingredients were separated well.The linear ranges of chlorogenic acid,caffeic acid,rutin,hyperoside,luteolin-7-O-glucoside and 4,5-O-dicaffeoylquinic acid were 3.24-64.80,0.24-4.79,1.22-24.40,0.09-0.44,0.68-13.55,1.19-23.87 mg·L-1 respectively.The average recoveries were above 97%（RSD3%,n=9）.Conclusion： This method is simple and accurate with good recovery and repeatability,and can be used in Jinqi Jiangtang tablets and the related preparation quality control.

关 键 词：高效液相色谱法 金芪降糖片 绿原酸 4 5-O-双咖啡酰奎宁酸 芦丁 木犀草苷 

分 类 号：R284.1[医药卫生—中药学]