山楂叶指纹图谱研究  

HPLC Fingerprint Study of Hawthorn Leaf

作　　者：王领弟[1] 李艳荣[1] 张晓峰[1] 潘海峰[1] 

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000

出　　处：《中国实验方剂学杂志》2011年第19期74-78,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：承德市科学技术研究与发展计划项目(20101312)

摘　　要：目的:研究不同产地山楂叶药材的HPLC指纹图谱并进行比较,同时建立绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、金丝桃苷和槲皮素的HPLC分析方法。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);乙腈-0.5%甲酸水溶液梯度洗脱,流速1.0 mL.min-1,检测波长350 nm,柱温30℃。对13批山楂叶药材进行了指纹图谱及含量测定研究,采用中药色谱指纹图谱相似度评价系统(2004A)软件进行分析。结果:绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、金丝桃苷和槲皮素的线性范围分别为3.75～120,9.69～310,24.69～790,6.25～200,0.31～10 mg.L-1(r分别为0.999 6,0.999 8,0.999 9,0.999 9,0.999 9);平均加样回收率分别为99.2%(RSD 1.7%),100.3%(RSD 1.8%),99.0%(RSD 1.4%),99.8%(RSD 2.1%),100.5%(RSD 1.9%);13批药材的HPLC指纹图谱共标定了15个共有峰,并对其中5个成分进行了含量测定。结论:该分析方法准确可靠,重复性好,为更好地控制山楂叶内在质量提供科学依据。Objective：To study the HPLC fingerprint of hawthorn leaf from different producing areas,establish a method for the analysis of chlorogenic acid,glucosyl-vitexin,vitexin-2″-O-rhamnoside,hyperoside and quercetin.Method： The separation was carried out on an Agilent ZORBAX SB-C18 column（4.6 mm×250 mm,5 μm）with the mixture of acetonitrile and 0.5% formic acid as mobile phase in a gradient elution model（flow rate 1.0 mL·min-1,detection wavelength 350 nm,column temperature 30 ℃）.13 groups of hawthorn leaf were analyzed with the developed HPLC fingerprint and determination methods,the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM（Version 2004A）.Result： The linear range of chlorogenic acid,glucosyl-vitexin,vitexin-2″-O-rhamnoside,hyperoside and quercetin were 3.75-120 mg·L-1（r=0.999 6）,9.69-310 mg·L-1（r=0.999 8）,24.69-790 mg·L-1（r=0.999 9）,6.25-200 mg·L-1（r=0.999 9）,0.31-10 mg·L-1（r=0.999 9）,respectively.The average recovery rates of chlorogenic acid,glucosyl-vitexin,vitexin-2″-O-rhamnoside,hyperoside and quercetin were 99.2%（RSD 1.7%）,100.3%（RSD 1.8%）,99.0%（RSD 1.4%）,99.8%（RSD 2.1%）,100.5%（RSD 1.9%）,respectively.In the fingerprint,15 common peaks of hawthorn leaf were confirmed and 5 peaks were determined.Conclusion：This method provides a scientific basis for controlling the quality of hawthorn leaf.

关 键 词：山楂叶 高效液相色谱 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]