苍耳子中酚酸类化合物的鉴别及绿原酸的含量测定  

Characterization of Phenolic Acids and Quantitative Analysis of Chlorogenic Acid Contained in Fructus Xanthii

作　　者：杨柳 吴金雄 许舜军[3] 苏芝军 曾星 黎雄 陈璐璐 

机构地区：[1]广州中医药大学第二附属医院 [2]广东省中医药科学院,广州510120 [3]澳门科技大学药物及健康应用研究所,澳门999078 [4]陕西省府谷县人民医院,陕西府谷719400

出　　处：《中国实验方剂学杂志》2011年第19期85-88,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:鉴别苍耳子药材中的酚酸类化合物,比较和分析不同产地苍耳子中绿原酸的含量,建立苍耳子的质量控制方法。方法:根据化合物的紫外光谱、液相色谱保留时间及质谱数据等综合信息鉴别苍耳子药材中的酚酸类成分,采用UPLC对苍耳子中绿原酸的含量进行测定,流动相甲醇-0.1%磷酸水溶液,检测波长为327 nm,流速为0.4 mL.min-1,柱温35℃。结果:对苍耳子药材中的9种酚酸类成分的化学归属进行了指认,并测定了24批苍耳子药材中绿原酸的含量,结果绿原酸在3.5～350 mg.L-1具有良好的线性关系(R2=0.999 9),平均回收率(n=6)为101.7%。结论:苍耳子中特征化学成分的识别可以显著增强其质量控制的准确性和专属性,而快速准确的指标成分含量测定方法,也是苍耳子质量控制的重要检测手段,定性与定量两方面结合,对于苍耳子药材及其相关产品的质量检测和控制具有重要意义。Objective：To assign phenolic acids and determine the content of chlorogenic acid contained in Fructus Xanthii collected from different product areas,and establish a practical quality control method for the herbal drug.Method： Major phenolic acids of this herb were identified based on their UV absorption,retention time and mass fragmentation characteristics.The content of chlorogenic acid was determined using ultra-performance liquid chromatography-ultraviolet detector（UPLC-UV）.The mobile phase consists of 0.1% phosphoric acid in water as solvent A and methanol as solvent B.The detection wavelength is set at 327 nm and the column temperature at 35 ℃.The mobile phase flow rate is 0.4 mL·min-1.Result： A total of 9 major chromatographic peaks were assigned as phenolic acids,and peak area vs.content of chlorogenic acid showed a good linearity in the range of 3.5-350 mg·L-1 with a correlation coefficient of 0.999 9 and the average recovery was 101.7%.Conclusion：The established method is suitable for determining the content of chlorogenic acid contained in Fructus Xanthii and can be used to effectively control the quality of the herb;and assigning major characteristic peaks can significantly enhance specificity and accuracy in accessing the quality of herb.

关 键 词：苍耳子 酚酸 鉴别 含量测定 超高液相色谱 

分 类 号：R284.1[医药卫生—中药学]