天麻饮片的HPLC指纹图谱鉴别  

HPLC Fingerprint Chromatograms for Processed Products of Gastrodia elata

作　　者：麻印莲[1] 肖永庆[1] 耿立冬[1] 张村[1] 李丽[1] 于定荣[1] 顾雪竹[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第19期104-107,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究建立天麻饮片的HPLC指纹图谱鉴别方法。方法:选择HPLC-UV指纹图谱法,Kromasil C18柱;流动相乙腈-0.5%冰醋酸溶液线形梯度洗脱,检测波长270 nm,流速1.0 mL.min-1。以指纹图谱软件计算相似度和图谱比较,以聚类分析比较不同来源饮片的差异。结果:建立了天麻饮片的标准指纹图谱,6个产地共10批次天麻饮片的指纹图谱基本一致,共能检出15个共有峰,10个样品的平均相似度>98%,但相对峰面积有较大差异;聚类分析结果与饮片产地基本一致。结论:该方法重复性好,结合聚类分析可用于天麻饮片的定性鉴别,为天麻饮片的质量控制提供了科学依据。Objective：To establish the HPLC fingerprint chromatogram identification of the processed products from Gastrodia elata.Method： HPLC procedure was performed on a chromatographic column of Kromasil C18.Acetonitrile-glacial acetic acid water solution（0.5%）was used as mobile phase by gradient elution,and the detection wavelength was at 270 nm.The mobile phase flow rate was at 1.0 mL·min-1.The similarity and the clustering analysis were carried out by fingerprint chromatogram software.Result： The standard HPLC Fingerprint chromatogram was established through the determination of 10 batches processed products from 6 different producing areas.15 peaks can be detected and the average similarity was over 98% in 10 samples.The processed products were almost the same on the whole chromatograms but the relative peak areas had the differences.The cluster analysis was consistent with the processed origins.Conclusion：HPLC method can be used in quality control of G.elata.

关 键 词：天麻饮片 指纹图谱 鉴别 

分 类 号：R284.1[医药卫生—中药学]