麝香乌龙丸对佐剂性关节炎大鼠滑膜病理形态及血管内皮生长因子、内皮抑素表达的影响  

Effect of Shexiang Wulong Pill on Pathomorphology and Vascular Endothelial Growth Factor,Endostatin of Synovium in Rats with Adjuvant Arthritis

作　　者：王志文[1] 丁春菊[1] 张爱国[1] 黄秀江[1] 李臣杰[1] 徐涛[1] 陈守中[1] 王鹏飞[1] 易永泽[1] 于荣霞[1] 

机构地区：[1]河北联合大学,河北唐山063000

出　　处：《中国实验方剂学杂志》2011年第19期241-244,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察麝香乌龙丸对佐剂性关节炎(AA)大鼠滑膜组织病变和血管内皮生长因子(VEGF)及内皮抑素(ES)的影响,探讨其作用机制。方法:建立大鼠AA模型,麝香乌龙丸低、高剂量(丸药1,2 g.kg-1),ig 1周后,观察滑膜组织病理形态学变化,免疫组化法检测各组大鼠滑膜组织中VEGF,ES的表达,以关节滑膜病理形态积分,VEGF,ES阳性细胞率,计算VEGF/ES比值。结果:麝香乌龙丸低、高剂量组均能减轻大鼠关节滑膜的病理积分,与模型组比较差异有统计学意义(P<0.05);低、高剂量组之间差异无统计学意义。麝香乌龙丸低、高剂量组均可降低大鼠滑膜VEGF含量,提高ES含量,降低VEGF/ES比值,与模型组比较差异显著(P<0.05或P<0.01);麝香乌龙丸低、高剂量组之间无统计学差异。结论:麝香乌龙丸可抑制AA大鼠炎症发展,其机制可能与麝香乌龙丸调控VEGF/ES的失衡而抑制滑膜组织血管生成相关。Objective：To observe the effects of Shexiang Wulong pill on pathomorphology and expression of vascular endothelial growth factor（VEGF） and endostatin（ES） of synovium tissue in the rats with adjuvant arthritis（AA）,and investigate Shexiang Wulong pill treatment for the rheumatoid arthritis whether concerns with the adjustment to the imbalance of VEGF/ES.Method：The rats with adjuvant arthritis model was established.Shexiang Wulong pill fills pill low dose and high dose（1,2 g·kg-1） were given orally.One week later,the structure changes of synovium tissue were observed,and the expression of VEGF and ES in synovium tissue was determined by immunohistochemical test.Result：The Shexiang Wulong pill low,high dose group both could reduce the pathological synovium integral,both dose group compared with model group had difference（P0.05）;the low dose group had no statistical difference with the high dose group.Compared with the model group,the low and high dose groups also could reduce the expression of VEGF,increase the expression of ES and reduce the ratio of VEGF/ES in synovium tissue（P0.05 or P0.01）.The low dose group had no statistical difference with the high dose group.Conclusion：The Shexiang Wulong pill can attenuate the degree of chronic inflammation and angiogenesis in synovium of AA rats.The mechanism might be associated with the adjustment to the imbalance of VEGF/ES in synovium of AA rats.

关 键 词：麝香乌龙丸 类风湿关节炎 佐剂性关节炎 血管内皮生长因子 内皮抑素 

分 类 号：R285.5[医药卫生—中药学]