大麻素WIN55,212-2对体外培养牛眼小梁细胞MMP-3、MMP-9及TIMP-1表达的影响  

Effect of WIN55,212-2 on the expression of mmp-3,mmp-9 and timp-1 in cultured bovine trabecular meshwork cells

作　　者：赵越超[1] 王万辉[2] 

机构地区：[1]山西医科大学,太原030001 [2]山西医科大学第二临床医院眼科

出　　处：《中国医疗前沿》2011年第14期20-22,共3页China Healthcare Innovation

摘　　要：目的研究大麻素WIN55,212-2对体外培养的牛眼小梁细胞MMP-3、MMP-9及TIMP-1表达的影响,从而探讨其降眼压机制。方法采用组织块培养法对牛眼小梁细胞进行原代及传代培养,应用免疫组化方法(NSE,Ⅷ因子相关抗原染色)鉴定细胞,应用透射电镜对细胞进行形态学及生长特性观察;对传3代的小梁细胞分别施加含大麻素WIN55,212-2终浓度为0(对照组)、1、10、20、40μmol/L的培养液,48h后行MMP-3和MMP-9免疫组化SP染色,结果进行计算机图像分析并进行统计学检验。提取上清液用ELASA法检测随浓度的不同TIMP-1量的变化。结果体外培养的牛眼小梁细胞表达MMP-3及MMP-9,大麻素WIN55,212-2可促进MMP-3及MMP-9的表达,并抑制TIMP-1的表达。结论一定剂量的大麻素可以促进牛眼小梁细胞MMP-3及MMP-9的表达(P<0.05),并抑制TIMP-1的表达(P<0.01),从而达到降低眼压的目的。Objective To study the effect of WIN55,212-2 on the expression of MMP-3,MMP-9 and TIMP-1 in cultured bovine trabecular meshwork cells（TMCs） and discuss its mechanism of reducing the intraocular pressure.Methods TMCs were obtained through the cultured tissue,bovine TMCs were prmiarily cultured and subcultured.We apply immunohistochemistry（NSE,Ⅷ factor related antigen staining） to identify the cell and apply transmission electron microscopy to observe morphology and growth characteristics of cells.The third passage cells were incubated with different dosages of WIN55,212-2（0,1,10,20 and 40μmol/L,final concentration,diluted by DMEM） for 48h.MMP-3 and MMP-9 were determined by immunohistochemistry method.The results were analyzed by the computer image-analysis system and statistical test.Levels of TIMP-1 in cell media were quantified by ELASA.Result The cultured bovine TMCs expressed MMP-3 and MMP-9.In comparison with the control group,WIN55,212-2 promoted the expression of MMP-3 and MMP-9,but inhibited TIMP-1 expression in bovine TMCs.Conclusion Certain concentration of WIN55,212-2 may promote the expression of MMP-3 and MMP-9 of TMCs（P0.05） and decrease TIMP-1 expression（P0.01）,so as to reduce the intraocular pressure.

关 键 词：大麻素WIN55 212-2 牛眼小梁细胞 基质金属蛋白酶 组织抑制因子 原发性开角型青光眼 

分 类 号：R339.1[医药卫生—人体生理学]