小鼠被动吸烟对脑部生物电和神经递质的影响初探  

THE PRELIMINARY STUDY OF THE INFLUENCE ON BRAIN EVOKED POTENTIAL AND THE EXPRESSION OF NEUROTRANSMITTERS AFTER MICE SMOKE EXPOSURE

作　　者：杨爱红[1] 李光武[2] 胡庆东[2] 

机构地区：[1]安徽淮北职业技术学院医学系,淮北235000 [2]安徽医科大学神经生物学研究所

出　　处：《现代预防医学》2011年第20期4225-4228,共4页Modern Preventive Medicine

基　　金：安徽省自然科学基金(090413145)

摘　　要：[目的]通过观察小鼠脑部电位和神经递质的变化,初步探讨其引发小鼠成瘾性及毒性的机制。[方法]造模后进行小鼠脑区定位,记录小鼠被动吸烟后脑区电位的变化并观察脑区神经递质的变化。[结果]1.诱发电位:(1)嗅球部诱发电位与自发电位比较:ETS刺激时,波形变化显著。(2)伏隔核部位诱发电位与自发电位比较:Nic刺激时,波形变化显著。(3)Nic刺激时,小鼠黑质部位诱发电位与自发电位比较,波形变化显著;ETS刺激时,小鼠黑质部位诱发电位与自发电位比较,波形变化显著。2.免疫组织化学结果:(1)GABA在小鼠大脑皮层、海马部位的表达,ETS暴露组阳性细胞数量明显减少(P﹤0.05)。(2)nAChR在大脑皮层的表达,ETS吸入组阳性表达明显增加(P﹤0.05)。(3)NMDAR在小鼠大脑皮层、纹状体皮质的表达,ETS吸入组、Nic吸入组阳性表达增加明显(P﹤0.05)。[结论]ETS、Nic可诱发小鼠嗅球、伏隔核、黑质等部位产生特征性OERP及导致大脑皮层、下丘脑等部位神经递质及受体表达的变化。[Objective] To set up an exposure model of and explore the mechanism of Environmental Tobacco Smoke and Major components leading to Addiction and Toxicity in Mice. [ MethOdS] 1. Localizing the region and recording the changes of potentials. 2. Testing the expression of Neurotransmitters. [ Results] 1.The results of Olfactory Event-related Potential （OERP） ： （1） The results of OERP on Olfactory bulb （OB） ： Waveforms significantly changed when the mice were forced to inhale ETS. （2） The results of OERP on Nucleus Accumbens septi （NA） ： Waveforms significantly changed when the mice were forced to inhale nicotine. （3） The results of OERP on Substantia Nigra （SN） ： Waveforms significantly changed when the mice were forced to inhale NiC or ETS, 2.The immunohistochemistry results： （1） the expression of GABA on the mice cerebral cortex （CC） and the hippocampus （Hip） could be observed much higher on the ETS-exposed group than the control group （P 〈 0.05）. （2） the expression of nAChR on CC could be found much higer on the ETS-exposed group than the control group （P 〈 0.05）. （3） the expression of NMDAR （Glu receptors） on the CC and striate cortex could be observed much higher on the ETS-exposed group, NIC-inhaling group and the control group （P 〈 0.05）. [ Conclusion ] Long-term ETS-exposed and NiC- exposed environment could induce specific OERP and the changes on the expression of neurotransmitter and receptors.

关 键 词：环境烟草烟雾 亚慢性吸入 成瘾 毒性 嗅觉相关电位 

分 类 号：R34[医药卫生—基础医学]