植物雌激素染料木黄酮与钙、维生素D_3联合预防去卵巢小鼠骨质疏松的作用  

Effects of phytoestrogen,genistein combined with calcium and vitamin D_3 on preventing osteoporosis in ovariectomized mice

作　　者：王芊[1] 张岩[1] 高璐[1] 薛延[1] 

机构地区：[1]北京市创伤骨科研究所,北京100035

出　　处：《卫生研究》2011年第5期587-590,共4页Journal of Hygiene Research

基　　金：北京市创伤骨科研究所所内课题

摘　　要：目的评价不同剂量植物雌激素染料木黄酮与钙、维生素D3联合应用预防卵巢切除小鼠骨质疏松的作用。方法 63只平均体重29g CD-1雌性小鼠随机分为7组,包括Sham组、卵巢切除(OVX)组、OVX给药组分为碳酸钙、维生素D3联合染料木黄酮的高剂量组(GH 67mg/kg)、中剂量组(GM 33.5mg/kg)、低剂量组(GL 16.75mg/kg)3组以及单纯染料木黄酮组、雌激素组(E2)。给药6周,测定小鼠骨密度(BMD)、骨矿含量(BMC)、骨生物力学和骨代谢生化指标。结果 GH、GM、GL组对卵巢切除小鼠的子宫有刺激生长作用,其中GL组作用小。GM组明显增加卵巢切除小鼠的BMD、BMC和股骨长度、股骨宽度,GL组明显增加卵巢切除小鼠的BMD(P<0.01)。GL组对卵巢切除小鼠的股骨最大载荷和最大应力升高最显著(P<0.01)。GL组骨碱性磷酸酶(BALP)明显升高、抗酒石酸酸性磷酸酶(TRAP)显著降低(P<0.01)。结论低剂量植物雌激素染料木黄酮与钙、维生素D3联合应用对卵巢切除小鼠的刺激子宫生长作用小。低剂量植物雌激素染料木黄酮与钙、维生素D3联合应用增加BMD,改善骨生物力学参数,促进骨形成和抑制骨吸收,降低了染料木黄酮的用量,并且较雌激素安全。Objective To evaluate the effects of different doses of phytoestrogen（genitein） combined with calcium and vitamin D3 on preventing osteoporosis in ovariectomized（OVX） mice.Methods 63 female CD-1 mice,29g average weight,were randomly divided into 7 groups.Including Sham group,OVX group and groups of treatment with calcium,vitamin D3 and genistein in high dose（GH,67mg/kg）,genistein in moderate does（GM,33.5mg/kg）,genistein in low dose（GL,16.75mg/kg）,pure genistein（G） and pure 17-βestradiol（E2）.After six weeks treatment,bone mineral density（BMD）,bone mineral content（BMC）,Biomechanical characteristics bone strength and bone biochemical markers were measured in all mice.Result In the groups of GH,GM and GL,it was stimulative effect of genistein on elevating uterine weight in ovariectomized mice and the effect in GL group is lower.BMD,BMC,length and width of femora were significantly increased in GM group mice than those in OVX mice（P0.01）,as well as BMD of femora in GL group mice were markedly increased（P0.01）.Peak load and resilience of femora were the most conspicuously increased in GL group mice than those in others treatment group mice（P0.01）.In GL group,bone alkaline phosphatase（BALP） increased and tartrate-resistant acid phosphatase（TRAP） decreased（P0.01）.Conclusion It was lower stimulative effect of low dose of phytoestrogen（genitein） combined with calcium and vitamin D3 on elevating uterine weight in ovariectomized mice.Low dose of Phytoestrogen（genistein） combined with calcium and vitamin D3 might increase BMD,improve the mechanical strength of bone,promote bone fromation and inhibit bone resorption significantly.It might reduce the dose of genistein administrated and be safter than E2.

关 键 词：染料木黄酮 骨质疏松 骨密度 骨强度 

分 类 号：Q946.885[生物学—植物学] R591.44[医药卫生—内科学]