2009-2010年上海市儿童呼吸道病毒病原谱的建立和分析  

Detection for respiratory viruses in Shanghai with multiplex PCR from 2009 to 2010

作　　者：赵百慧[1] 沈佳仁[1] 高烨[1] 俞雪莲[1] 张曦[1] 吴凡[1] 

机构地区：[1]上海市疾病预防控制中心,上海201306

出　　处：《卫生研究》2011年第5期635-637,共3页Journal of Hygiene Research

基　　金：国家"十一五"重大专项-传染病监测技术平台项目资助(No.2009zx10004-211);上海市科学技术委员会生物科技重大专项资助(No.09DZ1906601);上海市;区县疾病预防控制中心学科人才培养科研项目资助计划(中心2007-9)

摘　　要：目的应用多重PCR检测方法对上海市儿童呼吸道病毒感染进行检测,获得疾病谱分析其流行规律。方法 2009年1月到2010年3月上海市儿童医院因呼吸道感染住院病人下呼吸道标本519份,利用多重PCR技术进行12种常见呼吸道病毒的检测。结果在591例标本中病毒阳性率是58%(301/519)。其中呼吸道感染患儿中呼吸道合胞病毒B(RSVB)占首位,为37.5%(113/301),其次是鼻病毒(HRVA/B),为17.3%(52/301),比例最低的是副流感病毒2(PIV2),比例仅为0.7%(2/301)。人偏肺病毒(HMPV)的比例为6%(18/113)。结论 RSVB HRVA/B和PIV3以及Adv是上海市儿童呼吸道疾病感染的主要病原,混合感染以HRVA/B联合其他病毒为主。Objective To characterize the prevalence and occurrence of respiratory viruses in children in Shanghai China.Methods Respiratory virus were identified from aspirates and throat swabs selected from children with respiratory symptom who visited the Shanghai Children Hospital during period January 2009 to March 2010. Respiratory virus was detected by multiplex PCR.Results Virus were detected in 301 patients（58%）,among them.RSVB infection were the most frequent 37.5% of 301 patients.HRVA/B was found in 17.3%（52 patients）。Adv and PIV3 in 9%（27 patients）,HMPV in 6%（18 patients）.Conclusion The data indicate that RSVB HRVA/B and PIV3 Adv is an important etiological agent for respiratory infection in children during the survey period.HRVA/B combined other virus are the most virus for combined infection.

关 键 词：呼吸道病毒 多重PCR 

分 类 号：R188.3[医药卫生—流行病学] R373.1[医药卫生—公共卫生与预防医学]