综合评分法优选丝瓜络中利尿成分的提取工艺  

Optimization of Extraction Technology of the Diuretic Ingredients in Luffae cylindrica by Grading Methods

作　　者：房春燕[1] 孙文启 史立宏[1] 张秀荣[1] 王琳[1] 康白[1] 

机构地区：[1]潍坊医学院应用药理学实验室,潍坊市261053 [2]山东潍坊市皮肤病防治所,潍坊市261061

出　　处：《中国药房》2011年第39期3686-3687,共2页China Pharmacy

摘　　要：目的:优选丝瓜络中利尿成分的最佳提取工艺。方法:通过大鼠尿量选择合适的提取方法;以大鼠24h尿量、丝瓜络总提取物百分率的综合评分为评价指标,以乙醇浓度、回流时间和乙醇用量为影响因素进行正交试验,优选提取丝瓜络利尿成分的最佳工艺。结果:醇回流法为最合适的提取方法。乙醇浓度对评价指标有显著影响,回流时间及乙醇用量对评价指标无显著影响。最佳提取工艺为用70%的乙醇回流提取2次,第1次加8倍量乙醇提取1.5h,第2次加8倍量乙醇提取1h。结论:该方法简便、科学,可为开发丝瓜络的药理作用、临床应用提供理论依据。OBJECTIVE： To optimize the extraction technology of diuretic ingredients in Luffae cylindrica. METHODS： The appropriate method for the diuretic ingredients extraction was chosen. The extraction technology of diuretic ingredients in L. cylindrica was optimized by orthogonal experiment with urine volume and itegracted assessment of percent of extracts of L. cylindrica as index and with concentration of alcohol, the extracting time and the amount of alcohol as factors. RESULTS： Alcohol backflow technology was the most appropriate method. The extracting efficiency was affected by the concentration of alcohol significantly, and the influence of the extracting time and the amount of alcohol had no significance.The optimal process is as follows： extracting for 2 times in 70% alcohol, 8 folds alcohol, 1.5 hour for the first time, and 1 hour for the second time.CONCLUSION：The method is simple, science, and coulde be provided the theory basis for pharmacological effects and clinical application of L.cylindrica.

关 键 词：丝瓜络 提取 正交试验 综合评分法 

分 类 号：R285[医药卫生—中药学] R284.2[医药卫生—中医学]