紫外-可见分光光度法测定环草石斛中石斛多糖的含量  

Content Determination of Dendrobium Polysaccharides in Dendrobium loddigesii by UV-VIS Spectrophotometry

作　　者：陈化 陈竹[2] 梁斌 赵俊 冯子坤 

机构地区：[1]贵州兴义市人民医院,兴义市562400 [2]贵州省人民政府云岩宾馆医务室,贵阳市550004 [3]贵州兴义市吉仁堂药业公司,兴义市562400

出　　处：《中国药房》2011年第39期3690-3692,共3页China Pharmacy

基　　金：国家科技支撑计划子课题(2006BAI06A11-11)

摘　　要：目的:建立测定环草石斛中石斛多糖含量的方法。方法:采用紫外-可见分光光度法,以D-无水葡萄糖为对照,检测波长为490nm。结果:D-无水葡萄糖的进样量在0～0.10440mg范围内与吸光度呈良好的线性关系(r=0.9997);平均加样回收率为98.09%,RSD=1.62%(n=6)。结论:本法操作简便、快捷、结果准确,可用于环草石斛中石斛多糖的含量测定。OBJECTIVE： To establish a method for the content determination of dendrobium polysaccharides in Dendrobium loddigesii. METHODS： UV-VIS spectrophotometry method was adopted. The spectrophotometry was used with D-glucosum anhydricum as control. The detection wavelength was set at 490 nm. RESULTS： The linear range of D-glucosum anhydricum was 0- 0.104 40 mg（r=0.999 7）with an average recovery of 98.09%（RSD=1.62% ,n=6）. CONCLUSION： The method is simple, rapid and accurate for the content determination of dendrobium polysaccharides in D. loddigesii.

关 键 词：环草石斛 石斛多糖 D-无水葡萄糖 紫外-可见分光光度法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]