HPLC法测定杠板归不同药用部位槲皮素的含量  

Content Determination of Quercetin in Different Parts of Polygoni Polygonum perfoliatum

作　　者：李元宏[1] 杨务彬[1] 兰鸿[1] 

机构地区：[1]湖北医药学院附属太和医院药学部,十堰市442000

出　　处：《中国药房》2011年第39期3714-3715,共2页China Pharmacy

摘　　要：目的:对杠板归不同药用部位槲皮素含量进行测定。方法:采用高效液相色谱法。色谱柱为DiamonsilTMC-18(200mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(50:50),检测波长为360nm,流速为1.0mL·min-1。结果:槲皮素的进样量在0.05～1.00μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为97.73%,RSD=1.03%(n=6)。杠板归不同药用部位槲皮素含量差异较大。结论:本方法简便、准确、重复性好,可用于杠板归的质量控制。OBJECTIVE： To determinate the contents of quercetin in different parts of Polygonum perfoliatum. METHODS： HPLC was performed on Diamonsil^TM C-18 column （250 mm×4.6 mm, 5 μm）with methanol-0.4%phosphatic acid （50：50）as mobile phase at the flow rate of 1.0 mL·min ^-1. The detection wavelength was set at 360 nm. RESULTS： The linear range of quercetin was 0.05- 1.00 μg（r= 0.999 8） with an average recovery of 97.73 % （RSD = 1.03 %, n = 6）. The contents of quercetin were different significantly among different parts of P. perfoliatum.CONCLUSION： The method is simple, accurate and highly reproducible, and can be used for the quality control of P perfoliatum.

关 键 词：杠板归 药用部位 槲皮素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]