高效液相色谱-蒸发光散射检测法分析水产品中脑苷脂的含量  

Quantitative Analysis of Cerebrosides in Aquatic Products by HPLC Coupled with Evaporative Light-scattering Detection

作　　者：徐杰[1] 薛长湖[1] 冯婷玉[1] 丛培旭[1] 李兆杰[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2011年第18期286-290,共5页Food Science

基　　金：国家"863"计划项目(2007AA091805);"十一五"国家科技支撑计划项目(2008BAD94B05)

摘　　要：建立一种高效液相色谱-蒸发光散射检测法分析水产品中脑苷脂含量的方法。以TSKgel CN-80Ts色谱柱为分离柱,以正己烷-异丙醇和二氯甲烷-甲醇为流动相,采用梯度洗脱方式,流速1.0mL/min,漂移管温度50℃,氮气流速2.4L/min,选用海参、海胆、海星、鱼肝、鱼脑等水产品为研究对象。结果表明:在优化条件下14min内完成分离,脑苷脂的线性范围为0.05～5μg,检出限为0.01μg(RSN=3),相关系数>0.99,平均加标回收率为94.28%,相对标准偏差为4.90%,方法稳定性良好;在所测水产样品中,海星脑苷脂含量最高为11.21mg/g,海地瓜脑苷脂含量最低为0.35mg/g。该方法可行、快速简便、准确可靠,提示包括海参在内的水产品可以作为功能性鞘糖脂的潜在资源。A high performance liquid chromatography coupled with evaporative light-scattering detection（HPLC-ELSD） method for analyzing cerebroside content in aquatic products was developed.The separation was performed on a TSKgel CN-80Ts column by using hexane-2-propanol and dichloromethane-methanol as the mobile phase at a flow rate of 1.0 mL/min.The temperature of drift tube was kept at 50 ℃ and the flow rate of nitrogen was 2.4 L/min.The aquatic products sea cucumber,sea urchin,sea starfish,fish liver and fish brain were selected as experimental subjects.Under optimal conditions,effective separation of cerebrosides was completed within 14 min.The linear range of the established cerebrosides calibration curve was 0.05-5μg,and the limit of detection was 0.01μg with a regression correlation coefficient of 0.99.The average recovery rate for cerebrosides in sea cucumber across three spike levels was 94.28% with a relative standard deviation of 4.90%.An excellent stability of this method was observed.In these aquatic products,the content of cerebrosides in sea starfish was the highest,which was up to 11.21 mg/g;in contrast,sea cucumber had the lowest content of cerebrosides,which was 0.35 mg/g.These results demonstrate that the proposed method is feasible,rapid,simple and accurate.Meanwhile,aquatic products such as sea cucumber can be used as a potential source of functional sphingolipids.

关 键 词：高效液相色谱 蒸发光散射检测 脑苷脂 水产品 海参 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]