牛血吸虫病的快速粪便毛蚴孵化法  

A fast hatching test for Schistosoma japonicum miracidium in bufflo stool

作　　者：周伟芳 王禹斌[2] 金志恩 

机构地区：[1]上海奉贤区动物卫生监督所,上海201400 [2]苏州大学技术转化中心,苏州,215123

出　　处：《国际医学寄生虫病杂志》2011年第5期273-276,共4页International JOurnal of Medical Parasitic Diseases

摘　　要：目的比较3种孵化法检测牛血吸虫病的效果。方法将野外收集的血吸虫病阳性牛粪和实验室人工定量掺入虫卵的牛粪，分别用粪便铜丝筛淘洗尼龙兜过滤孵化法（常规法）、快速粪便毛蚴孵化法（快速法）、粪便双层尼龙兜淘洗孵化法（双层法）做病原学检测。结果快速法毛蚴相对孵出率为100％，而常规法为40．00％一81．86％，双层法为27．00％一63．16％。双层法的检出率最低，常规法和双层法差异无统计学意义。结论快速法可代替常规法用于牛血吸虫病的病原学检测。Objective To compare the effects of 3 hatching methods in detecting schistosomiasis of cattle. Methods Feces from schistosomiasis positive cattle or artificial mixed with Schistosoma japonicum eggs were used to exam S. japonicum miracidium in stool for evaluating fast hatching test, routine hatching test and double nylon hatching test. Results The hatchability rate of S. japonicum miracidium in stool with fast hatching test was 100% and that with the routine hatching test was 40.00% -81.86%, while with the double nylon hatching test was 27.00%-63.16%, respectively. Conclusion Fast hatching test is simpler and faster for examination of cattle schistosomiasis.

关 键 词：日本血吸虫病 快速孵化法 毛蚴 

分 类 号：R383.24[医药卫生—医学寄生虫学]