应用iTRAQ标记结合2D LC-MS/MS分析慢性苯中毒患者血清蛋白质组  

Quantitative proteomic analysis on serum in patients with chronic benzene poisoning using 8-plex iTRAQ labeling coupled with 2D LC-MS/MS

作　　者：黄振烈[1] 王海兰[1] 黄汉林[1] 夏丽华[1] 邱新香[1] 陈慈珊[1] 陈嘉斌[1] 陈甦生[1] 梁伟辉[1] 黄明[1] 郎丽[1] 郭义曹[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《中国职业医学》2011年第5期361-364,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30972459);广东省自然科学基金资助项目(8151030005000004);广东省医学科学技术研究基金项目(B2008005)

摘　　要：目的比较慢性苯中毒患者与健康人的血清蛋白质组,筛选差异表达蛋白质,为阐明其发病机制和寻找生物标志物提供靶标。方法免疫层析柱去除14种高丰度血清蛋白质后,应用8标iTRAQ技术结合2D LC-MS/MS分析并鉴定慢性苯中毒病例与健康人血清的差异表达蛋白质。结果对9例慢性苯中毒患者和9名健康人的血清蛋白样品的iTRAQ标记,分析并成功鉴定159种蛋白质,其中差异表达有统计学意义(P<0.05)的蛋白质有纤维蛋白溶酶原(PLG)、载脂蛋白B100(APOB100)、血小板碱性蛋白(PBP)。这些差异表达蛋白质具有结合、催化、酶调节和转运活性等分子功能,参与的生物过程包括免疫、代谢、应激、转运和凋亡等。结论 PLG、APOB100和PBP可能作为慢性苯中毒发病机制研究的候选靶标。Objective To compare the expression of serum proteins between patients with chronic benzene poisoning and healthy controls, and to screen the differentially expressed proteins for the study of molecular mechanisms and the development of biomarkers for treatment and prevention. Methods 14 kinds of high abundant proteins in the serum were removed by immunochromatography assay. Isobaric tags for relative and absolute quantitation （iTRAQ） coupled with two-dimensional liquid chroma- tography-tandem mass spectrometry （2D LC-MS/MS） were used to analyze and identify the proteins expression of serum. Re- sults 159 proteins were successfully identified from 18 samples of 9 patients and 9 controls. Statistic differences were observed in 3 proteins including plasminogen （PLG）, apolipoprotein B-100 （APOB100） and platelet basic protein （PBP）. The 3 proteins can be functionally categorised into binding, catalytic activity, enzyme regulator activity and transporter activity, as well as involving in biological processes of immunity, metabolism, stress, transportation and apoptosis. Conclusion PLG, APOB100 and PBP could serve as potential target molecules for the mechanism study on chronic benzene poisoning.

关 键 词：苯 ITRAQ 2D LC—MS/MS 血清 

分 类 号：R135.12[医药卫生—劳动卫生]