凋亡相关蛋白死亡受体5、Caspase-3和Caspase-8在煤工尘肺发生发展中意义  

Significance of apoptosis-related proteins in the occurrence and progression of coal workers' pneumoconiosis

作　　者：张春民[1,2] 白玉萍[1,2] 余艳琴[1,3] 陈志远[4] 张志浩[4] 陈刚[4] 徐应军[1] 沈福海[1] 姚三巧[1] 

机构地区：[1]河北联合大学公共卫生学院,河北唐山063000 [2]河北省煤矿卫生与安全实验室,063000 [3]内蒙古科技大学公共卫生学院,包头014000 [4]中国煤矿工人北戴河疗养院尘肺科,秦皇岛066000

出　　处：《中国职业医学》2011年第5期365-368,共4页China Occupational Medicine

基　　金：国家自然科学基金项目(30671741)

摘　　要：目的探讨煤工尘肺(CWP)患者肺泡巨噬细胞(AM)凋亡情况、凋亡蛋白的表达情况及其影响因素。方法选取24例接触煤尘或煤矽尘的观察对象和44例CWP患者为研究对象,收集其大容量肺灌洗回收液,分离纯化AM,离体培养24 h,苏木素-伊红(HE)染色,光学显微镜下观察AM的形态特征,用Western blot方法检测死亡受体(DR)5、Caspase-3和Caspase-8的相对水平。结果凋亡的AM染色质浓缩、边缘化、分割成块状;CWP患者AM的凋亡指数高于观察对象(P<0.05),且贰期患者高于壹期患者(P<0.05);观察对象DR5、Caspase-3和Caspase-8的表达量均低于CWP患者(P<0.05);接尘工龄≥20年组DR5、Caspase-3和Caspase-8的表达水平高于<20年组(P<0.05);发病工龄<10年组DR5和Caspase-3的表达水平高于≥10年组(P<0.05)。结论 DR5、Caspase-8和Caspase-3在CWP的发生发展中可能发挥着重要的作用,且凋亡蛋白的表达水平与接尘工龄、发病工龄有关。Objective To study preliminarily the expression of apoptosis-related proteins in alveolar macrophages （AM） derived from the patients of coal workers＇ pneumoconiosis （CWP） and to discuss the related-factors. Methods 24 observeds and 44 patients of CWP were chosen as the research subjects. AM obtained from the massive whole lung lavage fluid were purified and cultured for 24 hours in vitro, then stained by the method of HE. The shape of AM was observed by optical microscope. The relative expression of DR5, caspase-3 and caspase-8 were measured by the method of Western blot. Results Apoptotic AM showed typical morphological change under the optical microscopy. The apoptotic index of AM in CWP was higher than that in the observeds ; and the apoptotic index of AM at stage Ⅱ of CWP was higher than that at stage Ⅰ of CWP. The relative expressions of apoptosis-related proteins （ DRS, caspase-3 and caspase-8） of AM in CWP were higher than those in the observeds. The relative expression of apoptosis-related proteins of AM in the group of dust exposure （/〉20 years）were higher than those in the group of dust exposures （ 〈 20 years） ; The relative expressions of apoptosis-related proteins of AM in the group of incidence length of service （ 〈 10 years） were higher than those in group of incidence length of service （≥ 10 years）. Conclusion DRS, caspase-8 and caspase-3 may play an important role in the development of the CWP, and the level of apoptotic protein expression was related to the length of dust exposure and the length of service.

关 键 词：尘肺 肺泡巨噬细胞 凋亡蛋白 死亡受体5 

分 类 号：R135.2[医药卫生—劳动卫生]