羟基红花黄色素A缓解血小板激活因子诱导的内皮细胞炎症因子表达升高作用的研究  

Inhibitory effect of Hydroxysafflor Yellow A on endothelium inflammatory factors expression elevation induced by platelet activating factor

作　　者：金鸣[1] 裴崇强[1] 臧宝霞[1] 王麟[1] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所药理研究室,北京100029

出　　处：《心肺血管病杂志》2011年第5期429-432,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然科学基金资助项目(30572344);北京市自然科学基金资助项目(7072025)

摘　　要：目的:观察红花有效成分羟基红花黄色素A(HSYA)缓解血小板激活因子(PAF)诱发的内皮细胞炎症介质表达升高的作用。方法:培养Eahy926脐静脉内皮细胞株,加入HSYA,以PAF刺激后提取总RNA,RT-PCR法检测白细胞介素1-β(IL-1β)、IL-6、细胞间黏附分子-1(ICAM-1)及血管细胞黏附分子-1(VCAM-1)mRNA表达水平的变化。结果:PAF刺激后内皮细胞IL-1β、IL-6、ICAM-1及VCAM-1 mRNA表达水平升高,HSYA可缓解这种变化。结论:HSYA能够缓解PAF诱发的血管内皮细胞炎症介质IL-1β、IL-6、ICAM-1及VCAM-1 mRNA表达的升高。Objective：To observe the inhibitory effect of Hydroxysafflor yellow A（HSYA） on endothelium inflammatory factors expression elevation triggered by platelet activating factor（PAF）.Methods：Umbilicus vein endothelium cell line Eahy926 was cultured.HSYA and then PAF was added to this cell.After RNA isolation IL-1β,IL-6,ICAM-1 and VCAM-1 mRNA expression levels were assayed by RT-PCR technology with actin as internal control.Results：Endothelium IL-1β、IL-6、ICAM-1、VCAM-1 mRNA expression levels increased after PAF addition and HSYA can attenuate these changes.Conclusion：HSYA is effective to inhibit endothelium IL-1β,IL-6,ICAM-1 and VCAM-1 mRNA expression elevation induced by PAF.

关 键 词：羟基红花黄色素A 血小板激活因子 炎症介质 内皮细胞 

分 类 号：R54[医药卫生—心血管疾病]