高效液相色谱法测定鳙鱼肉中三种微囊藻毒素  

Study on simultaneous determination of three microcystins in Aristichthys nobilis by high-performance liquid chromatography

作　　者：陈海燕[1] 虞锐鹏[2] 俞幸幸[1] 俞明飞[1] 

机构地区：[1]浙江省宁波市鄞州区疾病预防控制中心,浙江宁波315100 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国卫生检验杂志》2011年第9期2152-2153,2156,共3页Chinese Journal of Health Laboratory Technology

基　　金：宁波市自然科学基金项目(2010A610041)

摘　　要：目的:建立能同时检测鳙鱼肉中三种微囊藻毒素(MC-RR、MC-YR、MC-LR)的高效液相色谱分析法。方法:鱼样经90%的甲醇提取、离心后经固相萃取柱富集、净化,甲醇洗脱,氮吹浓缩,以乙腈+水(含0.05%三氟乙酸)38+62为流动相,经C18柱分离2,38 nm波长检测。结果:三种微囊藻毒素在0.25 mg/L～2.50 mg/L均有良好的线性,其相关系数均大于0.9995;回收率在67%～83%;RSD均小于5.0%。结论:该法灵敏度高、操作简便、准确,适用于淡水鱼中微囊藻毒素的检测。Objective： To establish a method for the simultaneous determination of three microcystins（MCs）（MC-RR,MC-YR,MC-LR）in Aristichthys nobilis by high-performance liquid chromatography（HPLC）.Methods： MCs in Aristichthys nobilis were extracted by 90% methanol.The microcystins were determined by the method of HPLC after solid phase extraction（SPE）.The mobile phase is consist of 38% acetonitrile,62% water with 0.05% TFA.The microcystins were separated and determined with C18 column（250 mm×4.6 mm,i.d.,5 μm） by UV at 238 nm.Results： The three MCs in the range of 0.25 mg/L^2.50 mg/L had a good linear relation with correlation coefficient more than 0.9995.The recoveries were 67%～83% for the three MCs and RSDs were less than 5.0%.Conclusion： This method was found to be sensitive,accurate to determine trace MCs in freshwate fish.

关 键 词：微囊藻毒素 高效液相色谱法 固相萃取 鳙鱼 

分 类 号：O657.72[理学—分析化学]