反相高效液相色谱法测定食品中γ-氨基丁酸的研究  

Research on determination of γ-aminobutyric acid in food by RP-HPLC

作　　者：李遂勤 王立忠 彭海成[2] 

机构地区：[1]郑州铁路卫生监督所,郑州450052 [2]河南工业大学,郑州450052

出　　处：《中国卫生检验杂志》2011年第9期2179-2180,2182,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立反相-HPLC法测定食品中γ-氨基丁酸(GABA)的测定方法。方法:用正亮氨酸作为内标物,样品中的γ-氨基丁酸和正亮氨酸与24,二硝基氟苯(FDNB)反应转化成稳定的衍生物并用乙醚萃取,挥发后,残余物溶解在NaOH溶液中,处理液注入反相-HPLC中,用甲醇溶液在盐酸缓冲液中线性梯度洗脱,于400 nm处检测。结果:GABA、正亮氨酸分别和FDNB反应60 min后达到平衡,流出液检测峰位分别在45 min和60 min。GABA在1 ng/ml～100 ng/ml范围内具有良好的线性,其相关系数大于0.999,检出限为1 ng/ml,实际样品加标回收率为98.4±2.3%。结论:此方法简单、准确,用于实际样品检测,结果满意。Objective： To develop an analytical method to determine γ-aminobutyric acid（GABA） concentration in food using RP-HPLC.Methods： GABA in samples reacts with 1-fluoro-2,4-dinitrobenzene（FDNB） to form a stable derivative,which is extracted by ether.After evaporation,residue is re-dissolved in NaOH solution and injected into a RP-HPLC.The stable derivative is washed out in a methanol Tris-HCl gradient and detected at 400 nm.Isoleucine is used as an internal standard.Results： The derivatisation reactions with FDNB take 60 minutes to reach equilibriums for both GABA and isoleucine.The GABA-derivative and isoleucine-derivative have retention time of 45 min and 60 min respectively.There is a good linear relationship between peak area and GABA concentration in the range of 1 ng/ml^100 ng/ml.The linear correlation coefficient is over 0.999.The detection limit for GABA is 1 ng/ml.The recovery rate for food samples is 98.4＋2.3%.Conclusion： The analytical method described here is simple and accurate.It has been tested to analyse food samples with satisfactory results.

关 键 词：Γ-氨基丁酸 反相高效液相色谱 正亮氨酸 

分 类 号：O657.72[理学—分析化学]