高效液相色谱法测定人体血浆中的头孢噻利浓度  

HPLC determination of cefoselis in human plasma

作　　者：吴晓宇[1] 李招云[1] 高琳[1] 张学斌 

机构地区：[1]浙江省台州市中心医院检验科,浙江台州318000 [2]台州市药品监督局,浙江台州318000

出　　处：《中国卫生检验杂志》2011年第9期2259-2260,2262,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立高效液相-紫外(HPLC-UV)法测定人血浆中头孢噻利的浓度。方法:血浆样品经高氯酸沉淀蛋白处理后,用阿司匹林作为内标进行HPLC-UV检测;色谱柱采用Diamonsil C18(5μm,4.6 mm×150 mm)色谱柱;流动相为:乙腈+20 mmol/L醋酸铵(8+92,v/v),用冰醋酸调pH至5.20;检测波长为254 nm;结果:在选定的色谱条件下,头孢噻利与血液中的杂质分离良好。头孢噻利在血浆中的线性范围为0.2μg/ml～50.0μg/ml,最低检测浓度(LLOQ)为0.2μg/ml,日内和日间的精密度(RSD)均小于10%,准确度为96%～102%,提取回收率均大于80%。结论:该方法经考察符合生物样品的测定要求,可应用于人血浆中头孢噻利浓度的测定和药代动力学研究。Objective： To establish a HPLC-UV method for determining concentration of cefoselis in human plasma.Methods：The sample was deproteinated by perchloric acid and determined with HPLC-UV detection,the internal standard was aspirin;The analytical column was Diamonsil C18（5 μm,4.6 mm×150 mm） with the mobile phase consisted of acetonitrile and 20 mmol·L ammonium acetate（8∶92,v/v）.Then the samples were adjusted to pH5.20 by acetic acid;Detection was performed at wavelength of 254 nm.Results：Under the selected chromatographic conditions,cefoselis was departed from the endogenous compound of blood.The linear range of cefoselis were 0.2 μg/ml to 50.0 μg/ml in plasma,minimum detectable concentration was 0.2 μg/ml,the inter-and intra day precision（RSD） were less than 10%,with accuracy of 96% to 102% and the extraction recovery of cefoselis from plasma was 80%.Conclusion：This specific,sensitive and precise method is suitable for monitoring of cefoselis in human and its pharmacokinetic investigation.

关 键 词：头孢噻利 高效液相色谱法 血药浓度 

分 类 号：O657.72[理学—分析化学]