聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法改良  

Improvement of Polyacrylamide Gel Electrophorus in Phytophthora infeatans SSR Marker

作　　者：张芳[1] 权军利[1] 单卫星[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《安徽农业科学》2011年第27期16606-16607,共2页Journal of Anhui Agricultural Sciences

基　　金：国家公益性行业科研专项资助项目(3-20);现代农业产业技术体系资助项目(nycytx-15)

摘　　要：[目的]改进聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法。[方法]以2009年在宁夏固原地区采集的马铃薯晚疫病样为试验材料,提取其DNA,PCR扩增,并将其产物进行聚丙烯酰胺凝胶电泳。[结果]采用12%聚丙烯凝胶电泳检测引物D13、G11,8%聚丙烯凝胶电泳检测引物PI4B、PI63、SSR4,最后采用0.1%硝酸银染色,取得了比较理想的电泳及染色效果。[结论]该研究建立的适用于致病疫霉菌SSR标记的电泳和染色技术方法具有检测灵敏度高、操作简单、获得的条带清晰等特点,是一种行之有效、简便快捷的检测方法。[Objective] The paper was to improve polyacrylamide gel electrophorus in Phytophthora infeatans SSR Marker.[Method] With the disease sample of P.infeatans collected from Guyuan in Ningxia Province in 2009 as test material,its DNA was extracted and amplified with PCR,and its products were detected by polyacrylamide gel electrophoresis.[Result] 12% polyacrylamide gel electrophoresis was used to detect primers D13,G11,and 8% polyacrylamide gel electrophoresis was used to detect primers PI4B,PI63,SSR4,then 0.1% silver nitrate was used to stain,and an ideal electrophoresis and staining effect was obtained.[Conclusion] The electrophoresis and staining method suitable for P.infeatans SSR Marker established in the study had the characteristics of high sensitivity,simple operation and clear bands,which was an effective,simple and quick detection method.

关 键 词：聚丙烯酰胺凝胶电泳 致病疫霉 SSR 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]