葱蒜锈病叶的红外光谱研究  

Infrared Spectroscopic Study of Allium fistulosum and Allium sativum Rust Leaves

作　　者：何志遥[1] 刘海丽[1] 吴秋娟[1] 周湘萍[1] 刘刚[1] 董勤[2] 俞帆[2] 

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明650092 [2]云南大学实验中心,云南昆明650091

出　　处：《安徽农业科学》2011年第27期16659-16660,16713,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30960179)

摘　　要：[目的]采用傅里叶变换红外光谱对葱蒜锈病叶进行了研究,探讨中红外光谱在作物病害诊断中的应用潜力。[方法]用傅里叶变换红外光谱测试了葱蒜正常绿叶及锈病叶,分析其光谱差异。[结果]葱、蒜绿叶的红外光谱主要显示了多糖类物质、蛋白质、酯类振动;正常绿葱蒜叶和锈病葱蒜叶光谱在1 800～1 000 cm-1指纹范围显示了差异,与正常绿叶相比,锈病葱叶在1 640 cm-1的酰胺Ⅰ振动峰变强,在1 103 cm-1处的肩峰不明显;锈病叶的吸收强度比A1640/A1063、A1640/A1736、A1640/A2924和A1063/A2924较正常叶相应比值A1638/A1059、A1 638/A1 738、A1 638/A2 922和A1 059/A2 922变大。正常蒜叶的多糖振动峰1 056 cm-1为指纹区的最强峰,而锈病叶在1 634 cm-1处峰为指纹区的最强峰;锈病蒜叶的吸收强度比A1634/A1069、A1634/A1099、A1409/A2923和A1634/A1737较正常叶的相应比值A1627/A1056、A1627/A1104、A1411/A2920和A1 627/A1 740变大,锈病叶的A1 634/A2 923、A1 069/A2 923和A1 737/A2 923较相应正常叶的A1 627/A2 920,A1 056/A2 920,A1 740/A2 920变小。[结论]根据红外光谱差异可以区分绣病叶与正常叶,红外光谱可望发展成为作物病害检测的方法。[Objective] Fourier transform infrared spectroscopy was used to study Allium fistulosum and Allium sativum rust leaves,so as to explore the application potential of mid-infrared spectroscopy in the diagnosis of crop disease.[Method] The normal green leaves and rust leaves of A.fistulosum and A.sativum were tested using fourier transform infrared spectroscopy,and their spectral differences were analyzed.[Result] The infrared spectra of green leaves of A.fistulosum and A.sativum mainly demonstrated the vibrational bands of polysaccharides,protein and ester.Normal and rust leaves exhibited differences in the fingerprint interval of 1 800-1 000 cm-1,in which the band at 1 640 cm-1 in the spectrum of A.fistulosum rust leaf become stronger,and the band at 1 103 cm-1 was not obvious compared with the spectrum of normal leaf.The absorbance ratios A1 640/A1 063,A1 640/A1 736,A1 640/A2 924,and A1 063/A2 924 of A.fistulosum rust leaf were larger than the corresponding ratios A1 638/A1 059,A1 738/A1 638,A2 921/A1 638,and A2 921/A1 059 in the spectrum of normal leaf.The band of polysaccharides at 1 056 cm-1 in the spectrum of A.sativum normal leaf was the strongest in fingerprint region,while the band at 1 634 cm-1 in the spectrum of A.sativum rust leaf was the strongest in that area.The absorbance ratios A1 634/A1 069,A1 634/A1 099,A1 409/A2 923,and A1 634/A1 737 of A.sativum rust leaf were larger than the corresponding ratios A1 627/A1 056,A1 627/A1 104,A1 411/A2 920 and A1 627/A1 740 of normal leaves.While the ratios A1 634/A2 923,A1 069/A2 923,and A1 737/A2 923 of A.sativum rust leaf were less than the corresponding values of A1 627/A2 920,A1 056/A2 920,and A1 740/A2 920 of normal leaf.[Conclusion] Rust leaf and normal leaf could be distinguished according to the differences in infrared spectra,and infrared spectroscopy could be developed as a detection method for crop diseases.

关 键 词：葱叶 蒜叶 锈病 傅里叶变换红外光谱 

分 类 号：O657.33[理学—分析化学]