营养条件对Chlorella vulgaris生长的影响  

EFFECT OF NUTRIENT CONDITION ON THE GROWTH OF Chlorella vulgaris

作　　者：李超[1] 冯玉杰[1] 张大伟 

机构地区：[1]城市水资源与水环境国家重点实验室(哈尔滨工业大学),哈尔滨150090 [2]哈尔滨工业大学(威海)环境科学与工程系,威海264200

出　　处：《太阳能学报》2011年第8期1134-1138,共5页Acta Energiae Solaris Sinica

基　　金：城市水资源与水环境国家重点实验室自主课题(2008DX01)

摘　　要：以Chlorella vulgaris为研究对象,考察了培养基和有机碳源的影响。结果表明:在不同的营养方式中,混养的细胞密度和比生长速率最大,均超过自养和异养之和;改变培养液中的有机碳源种类,C.vulgaris的细胞密度最大分别达到2.92g/L(葡萄糖)、1.62g/L(乙酸钠)和1.05g/L(乙醇),且以葡萄糖为碳源时的比生长速率最大;当葡萄糖浓度从5g/L增加到30g/L时,C.vulgaris的比生长速率由0.0248h^(-1)降低至0.0186h^(-1),不过在培养晚期,C.vulgaris在10g/L葡萄糖时的细胞密度超过5g/L葡萄糖时的密度;当以10g/L葡萄糖作为C.vulgaris混养的有机碳源时,BG11培养基的细胞密度和比生长速率最大,分别达到3.41g/L和0.0257h^(-1),而在Basal培养基中,C.vulgaris培养液较稳定,藻细胞始终处于悬浮的状态,其细胞密度和比生长速率分别为3.25g/L和0.0204h^(-1)。Effect of culture medium and organic carbon source to growth of Chlorella vulgaris were investigated. The results show that both the cell density and the specific growth rate of mixtrophic algae were the largest in three metabolic pathways, either the cell density or the specific growth rate was larger than the sum of autotrophic and heterotrophic. When mixtrophic C. vulgaris was cultured using different organic carbon source, the maximal cell density was 2.92g/L （ glucose）, 1 the specific growth rate reached the 62g/L （sodium acetate） and 1.05g/L （alcohol）, respectively. In addition, largest when glucose was used. However, the specific growth rate of C. vulgaris decreased from O. 0248h-1 to 0. 0186h-1 as the concentration of glucose increased from 5 to 30g/L. It was found that the cell density at lOg/L glucose exceeded 5g/L glucose at stable period of growth. The maximal cell density and specific growth rate of C. vulgaris were achieved when the BG11 medium was used and 10g/L glucose added. It was 3.41g/L and 0.0257h -1, over the culture period. Then respectively. When the Basal medium was used, the cells of algae were suspended the cell density and specific growth rate was 3.25g/L and 0. 0204h -1, respectively.

关 键 词：CHLORELLA VULGARIS 有机碳源 细胞密度 

分 类 号：Q948.1[生物学—植物学]