溴吡斯的明白蛋白微球的制备  

Preparation of albumin microspheres containing pyridostigmine promide

作　　者：李艺[1] 谭群友[2] 柳珊[1] 徐美玲[1] 赵春景[3] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆400016 [2]第三军医大学大坪医院野战外科研究所胸外科,重庆400042 [3]重庆医科大学附属第二医院药剂科,重庆400010

出　　处：《中国医院药学杂志》2011年第19期1567-1570,共4页Chinese Journal of Hospital Pharmacy

基　　金：教育部博士点基金资助项目(编号:20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助;编号:KJ090308)

摘　　要：目的:制备溴吡斯的明白蛋白微球,并对其形态学性质、载药量、包封率进行考察。方法:采用乳化-固化法制备溴吡斯的明白蛋白微球,以包封率和载药量作为考察指标,进行正交设计试验优化工艺。结果:最佳工艺条件为药量:牛血清白蛋白量为1∶3,固化时间8 h,戊二醛饱和的甲苯溶液用量0.2 mL。所得微球包封率为(87.1±2.4)%,载药量为(21.8±0.6)%,算术平均径21.62μm,跨距为0.791μm。结论:本法工艺稳定可行,重复性好,采用该最佳工艺制备的溴吡斯的明白蛋白微球外观圆整,包封率及载药量高。OBJECTIVE To prepare the pyridostigmine bromide-bovine serum-albuminmicrospheres and to study the properties of its morphology, drug contents and drug loading. METHODS The pyridostigmine bromide-bovine serum-albumin-mi- crospheres was prepared by emulsion cross-linking method. The optimum preaparation condition was studied by orthogonal test with the encapsulation efficiency and drug loading rates as the index of evaluation. RESULTS The optimum preparation condition was as follows： drug： BSA was 1：3, the solidified time was eight hours, the usage of glutaraldehyde-saturated toluene was 0. 2 mL. The drug loading rate of the microspheres was （21.8 ± 0. 6） %, the encapsulation efficiency was （87. 1 ± 2. 4） %, and the arithmetic mean diameter was 21.62 μm, the span was 0. 791μm. CONCLUSION The preparation technique is stable, feasible and reproducible. The microspheres made by the optimum preparation is regular in its morphology and have a high encapsulation efficiency and drug loading rate.

关 键 词：溴吡斯的明 白蛋白微球 正交试验 

分 类 号：R943[医药卫生—药剂学]