枇杷试管苗限制生长保存过程中叶肉细胞超微结构变化的研究  

Ultrastructural Changes of Mesophyll Cells during Restricting Growth Preservation in vitro Plantlets of Loquat(Eriobotrya japonica)

作　　者：周红玲[1,2] 郑加协[1] 赖钟雄[2] 陈石[1] 

机构地区：[1]福建省农业科学院闽台园艺研究中心,福建漳州363005 [2]福建农林大学园艺学院亚热带果树研究所,福建福州350002

出　　处：《热带作物学报》2011年第8期1479-1482,共4页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划(No.2007BAD07B01);福建省重大科技专项(No.2004NZ 02-2)

摘　　要：本试验以早钟6号枇杷试管苗为材料,将其分别保存在MS+5 mg/L PP333培养基上和MS培养基(对照)上,采用超薄切片透射电镜对保存的试管苗成熟叶片叶肉细胞进行观察。结果表明:与对照相比,添加5 mg/L的PP333,保存6个月时,明显推迟了叶绿体的变形、移动、叶绿体和线粒体的镶嵌及叶绿体的解体;保存9个月时,叶肉细胞叶绿体结构完整,少数出现解体,淀粉粒明显增大、增多,线粒体和细胞核结构正常。The ultrastruetural changes of the mesophyU cells in response to in vitro plantlets of the loquat cuhivar Zaozhong No.6 preserved on MS＋5 mg/L PP333 were investigated. The results showed that the deformation, shift and disintegration of chlorolplasts and the mosaic of mitochondria were significantly delayed during restricting growth preserved for 6 months. The structure of the chloroplasts in the mesophyll cells was intact, only a few of that disintegrated. The starch grain significantly became larger and numerous. The structure of mitochondria and cell nuclear was normal when preserved for 9 months.

关 键 词：枇杷 限制生长保存 叶肉细胞 叶绿体 线粒体 超微结构 

分 类 号：S667.3[农业科学—果树学]