巴西橡胶树病程相关蛋白基因(HbPR1)的克隆及分析  

Cloning and Expression Analysis of Gene Encoding Pathogenesis-Related Protein1 in Hevea brasiliensis

作　　者：罗红丽[1] 秦云霞[2] 闫志烨[1] 向鹏[1] 

机构地区：[1]海南大学海南省热带作物资源可持续利用重点实验室,海南海口570228 [2]中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带作物学报》2011年第8期1499-1502,共4页Chinese Journal of Tropical Crops

基　　金：农业部橡胶树生物学重点实验室--海南省热带作物栽培生理学重点实验室开放课题项目(No.KLOF1101);海南省自然科学基金项目(No.311030)

摘　　要：利用同源克隆技术,在前期课题组对橡胶树转录组进行测序的基础上,根据模式植物中PR1蛋白的氨基酸序列进行同源搜索并设计引物,通过RT-PCR方法扩增巴西橡胶树PR1同源基因的cDNA片段,命名为HbPR1。该片段包含一个450 bp的开放阅读框,编码150个氨基酸的多肽。生物信息学分析结果表明,该氨基酸序列与杨树(Populus tomentosa)、葡萄(Vitis vinifera)和拟南芥(Arabidopsis thaliana)的PR1蛋白高度同源,同源性分别为76%,74%和72%,并含有SCP_PR1_like结构域。半定量分析结果表明,该基因表达受水杨酸的诱导。以上结果暗示该基因为橡胶树的PR1同源基因,可作为橡胶树系统获得性抗性产生的标签基因。Based on the result from the sequence of PR1 in Arabidopsis thaliana and the data of RNA-Seq analysis in Hevea brasiliensis, the homolog of rubber PR1 was cloned by RT-PCR and named as HbPR1. The sequencing result showed that the open reading frame of HbPR1 was 450 bp, encoding a 150 amino acids with a conserved SCP_PRI_like domain similar with PRIs from other plants. Bioinformatics analysis showed that the amino acid sequence of the HbPR1 was 76%, 74% and 72% similar with that of PRls from Populus tomentosa, Vitis vinifera and Arabidopsis thaliana. The expression of HbPR1 was induced by SA at transcript level. All the data indicated that HbPR1 was the PR1 homolog and could be used as a gene marker for SAR in rubber.

关 键 词：巴西橡胶树 系统获得性抗性 病程相关蛋白1(PR1) 

分 类 号：S794.1[农业科学—林木遗传育种]