香蕉枯萎病菌esyn1基因的克隆与序列分析  

Cloning and Sequence Analysis of esyn1 Gene in Fusarium oxysporum f. sp. Cubense

作　　者：陈石[1,2] 李春雨[2] 易干军[2] 陈丽娜[3] 周红玲[1] 郑加协[1] 

机构地区：[1]福建省农业科学院闽台园艺研究中心,福建漳州363005 [2]广东省农业科学院果树研究所,广东广州510640 [3]天福茶学院,福建漳州363202

出　　处：《热带作物学报》2011年第8期1503-1506,共4页Chinese Journal of Tropical Crops

基　　金：农业部"948"项目(2011-G16);福建省农业科学院青年创新基金(2010QA-2);国家自然科学基金(30971991)

摘　　要：采用RT-PCR方法扩增了香蕉枯萎病菌恩镰孢菌素合成酶基因esyn1的cDNA片段,测序结果表明,esyn1基因核苷酸序列阅读框架全长546 bp。核苷酸序列分析显示该基因片段编码181个氨基酸,推测分子量为19.9 ku,等电点为5.06。氨基酸序列比对结果表明,它与半裸镰刀菌、层出镰刀菌、拟枝孢镰刀菌esyn1基因的氨基酸序列的相似性分别为96%、94%、77%。The esynl gene, encoding a muhifunctional enzyme enniatin synthetase, was amplified by Reverse Transcription Polymerase Chain Reaction. Sequence analysis showed the sequence of esynl gene was 546 bp and it encoded 181 amino acids, the molecular weight and theoretical isoelectric point was 19.9 ku and 5.06, respectively. The nucleotide identities of esynl compared with Fusarium proliferatum, Fusarium pallidoroseum and Fusarium poae, was 96%, 94% and 77%, respectively. These results would help to understand the function of esynl genes in infection process.

关 键 词：尖孢镰刀菌古巴专化型 esyn1基因 序列分析 

分 类 号：S668.1[农业科学—果树学] S432.4[农业科学—园艺学]