细胞色素C在2-硫脲嘧啶修饰金电极上的直接电化学  

Direct Electrochemistry of Cytochrome C at 2-Thiouracil Modified Gold Electrode

作　　者：白林山[1] 韩睿[1] 吕丹丹[1] 

机构地区：[1]安徽工业大学化学与化工学院,安徽马鞍山243002

出　　处：《安徽工业大学学报（自然科学版）》2011年第4期375-379,共5页Journal of Anhui University of Technology（Natural Science）

基　　金：安徽工业大学研究生创新研究基金项目(2010009)

摘　　要：研究2-硫脲嘧啶(2-thiouracil,TU)修饰金电极的制备方法及其对细胞色素C直接电化学反应的促进作用。循环伏安实验表明,细胞色素C在2-硫脲嘧啶修饰金电极上的氧化还原反应是扩散控制的准可逆过程,峰电流与扫描速度的平方根呈线性关系,峰电位差(ΔE)为68 mV(vs.SCE),峰电流比接近于1,电子迁移速率常数为3.79×10-4 cm/s;2-硫脲嘧啶修饰金电极对细胞色素C直接电化学反应的促进作用稳定性较好,且与2-硫脲嘧啶在金电极上吸附量有关。实验结果对于进一步研究开发新型生物电化学传感器具有重要意义。The preparation of 2-thiouracil（TU） modified gold electrode and its promotion to the direct electrochemical behavior of cytochrome C were studied.The results of cyclic voltammetry indicated that the direct electron transfer reaction of cytochrome C at 2-thiouracil modified gold electrode was diffusion controlled quasi-reversible process,and the peals currents were linear correlated to the square root of scan rate.The separation of peak potentials was 68 mV（vs.SCE）,and the ratio of peak currents was approximately 1.The electron transfer rate coefficient was 3.79×10-4 cm/s.The promotion of 2-thiouracil modified gold electrode to the direct electrochemical behavior of cytochrome C was stable,and it was related to its adsorptivity on gold electrode.The experimental results are valuable for further development of bioelectrochemical sensors.

关 键 词：细胞色素C 2-硫脲嘧啶 化学修饰电极 循环伏安法 单分子自组装膜 

分 类 号：O657.1[理学—分析化学]