丰富环境干预对短暂性全脑缺血大鼠学习记忆能力的影响  

The effect of enriched rehabilitative training on cognitive impairment in rats after transient global cerebral ischemia

作　　者：刘海燕[1] 蔺勇[2] 张扬[3] 程永杰[2] 

机构地区：[1]北华大学附属医院,吉林吉林132001 [2]长春市中心医院,吉林长春130051 [3]吉林大学药学院,吉林长春130021

出　　处：《中风与神经疾病杂志》2011年第9期820-822,共3页Journal of Apoplexy and Nervous Diseases

基　　金：吉林市科委资助项目(201032226)

摘　　要：目的探讨丰富环境干预对短暂性全脑缺血大鼠学习记忆能力的影响。方法本研究将Wistar大鼠随机分为缺血组和假手术组,采用四血管阻断法(4-VO)建立了大鼠短暂性全脑缺血再灌注模型。造模后缺血组随机分为丰富环境组(IE)和标准环境组(IS),假手术组亦随机分为丰富环境组(SE)和标准环境组(SS)。术后3d,IE组SE组置于丰富环境笼饲养,IS组和SS组置于标准笼饲养。于干扰14d(即缺血28d)开始,采用Morris水迷宫测评实验大鼠学习和记忆能力。结果经过4d的认知训练后我们发现,与IE组和假手术组相比,IS组动物找到平台的逃避潜伏期延长,经由路线增加(P<0.05)。IE组和假手术组之间比较,上述改变趋势基本一致,缺乏统计学上的显著性(P>0.05)。空间探索实验中的运动轨迹显示,在撤除平台后IE组和假手术组均能记忆原平台的位置,寻找路线大多集中在原平台象限,平台对面象限最少,而IS组路线较散乱,运动轨迹以随机式和边缘式为主。结论丰富环境干预可以改善脑缺血大鼠的学习记忆能力。Objective To study the effect of enriched rehabilitative training on cognitive impairment in rats after transient global cerebral ischemia.Methods Adult male Wistar rats were used in the study and transient global cerebral ischemia was induced by the four-vessel occlusion method while control animals were subjected to anesthesia and sham surgery alone.Within 3 days after surgery,rats were randomly assigned to either enriched environment housing or standard condition.All animals were sacrificed 14 days after behavioral training.The learning and memory ability was evaluated by Morris water maze.Results Even though all animals learned to perform the water maze task over time,the standard condition group took longer to learn the task while the enriched environment group performed as well as the sham groups.Conclusion Enriched rehabilitative training can improve the ability of learning and memory.

关 键 词：丰富环境 可塑性 认知障碍 

分 类 号：R493[医药卫生—康复医学] R743.3[医药卫生—临床医学]