不同来源沙门菌入侵基因invH的检测及序列分析  

Sequence analysis and detection of invH gene from separate sources of Salmonella spp.

作　　者：王小龙[1] 牛星 张德庆[1] 夏庆祥[1] 牛钟相[1] 

机构地区：[1]山东农业大学动物医学院山东省动物生物技术与疫病防治重点实验室,山东泰安271018 [2]山东省畜牧兽医职业学院,山东潍坊261061

出　　处：《中国兽医学报》2011年第10期1449-1452,共4页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑计划资助项目(2006BAD06A11)

摘　　要：根据沙门菌invH基因序列设计1对引物,对9株沙门菌和4株常见病原菌进行PCR扩增,并将扩增出的in-vH基因进行克隆及序列分析,应用生物信息学方法预测invH蛋白的T细胞抗原表位。结果表明,9株沙门菌PCR均扩增出519bp的特异条带,4株非沙门菌均无特异带扩增;核苷酸序列分析证实,3株鼠伤寒沙门菌之间同源性为99.8%,与鸡白痢和鸡肠炎沙门菌之间的核苷酸序列同源性为98.6%,3株猪伤寒沙门菌和3株鸡源性沙门菌同源性较低,且不在同一支系统进化树上;invH蛋白有5个重复率最高的T淋巴细胞受体识别的抗原表位。A pair of oligonucleotide primer was synthesized according to the invH gene nucleotide sequence of Salmonella spp..And the invH gene was amplified from 9 strains of Salmonella and other 4 strains of pathogenic bacterium by PCR.Then the amplified invH gene was also cloned and sequenced.Based on bioinformatic techniuque,the antigenicity of T lymphcytes on invH protein was screened.The results showed the 519 bp specific bands was amplified from 9 strains of Salmonella by PCR,and no specific product from other 4 strains of pathogenic bacterium.The homology among three strains of S.typhinurium was 99.8%,among the three strains of S.typhinurium and Salmonella pollorum and Salmonella enteritidis was 98.6%.But the homology of the three S.typhisuis and three chicken-derived Salmonella was low,and the two derivations of Salmonella were not in the same phylogenetic tree.There were five higest repeated T cell epitope on invH protein.

关 键 词：沙门菌 invH基因 序列分析 抗原表位 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]