不同生长阶段猪小肠上皮细胞增生和凋亡指数的变化规律  

Variation on enterocyte apoptosis and proliferation index of pigs at different growth phases

作　　者：赵元[1,2] 秦贵信[1,2] 张兵[2] 王涛[2] 王波[2] 孙泽威[2] 鲍男[1,2] 

机构地区：[1]吉林农业大学动物生产及产品质量安全教育部重点实验室,吉林长春130118 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国兽医学报》2011年第10期1476-1479,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金重点资助项目(30430520);吉林省教育厅"十一五"科学技术研究资助项目(201044);吉林省科技厅重大科技攻关资助项目(09ZD6G008)

摘　　要：选取军牧一号29日龄(7.06±0.18)kg、99日龄(44.54±9.0 4)kg和169日龄(78.93±7.37)kg去势公猪各5头,分别进行仔猪、生长猪和肥育猪3个生理阶段的动物试验。仔猪于28日龄断奶,每一阶段试验期为7d。试验期结束后屠宰,迅速采集十二指肠、空肠中段和回肠组织样品。采用Ki-67抗体或TUNEL免疫组织化学染色方法分别检测小肠上皮细胞增生或凋亡情况,同时测定小肠绒毛高度和隐窝深度。结果表明,随着猪日龄的增长,小肠绒毛高度和隐窝深度增大(P<0.01);肠上皮细胞凋亡和增生指数逐渐降低(P<0.001)。无论哪一生长阶段的猪,不同肠段上皮细胞凋亡和增生指数有着相同的变化规律,空肠中段最高,回肠最低(P<0.01)。Five weanling（7.06±0.18） kg,5 growing（44.54±9.04） kg,or 5 finishing（78.93±7.37） kg General No.1 barrows,weaned on the 28 days of age,were randomly allotted to three experiments with five replicates for different growth phases,respectively.All the experimental periods were followed for 7 days.The tissue of duodenum,middle jejunum and ileum of pigs at different growth phases were collected,5 μm thick sections of small intestinal tissue were stained with the TUNEL method to assess apoptotic activity,and with Ki-67 immunohistochemistry to assess cellular proliferation.Meanwhile,villus height and crypt depth were determined.The results indicated that with the aging of pigs,the proliferative index and apoptotic index significantly decreased（P〈 0.01）,and the villus height and crypt depth increased（P 〈0.01）.The apoptotic and proliferative indexes had the same variation for piglets,growers and finishers（P〈0.01）,the highest in the mid-jejunum and the lowest in the ileum.

关 键 词：猪 增生指数 凋亡指数 肠上皮细胞 

分 类 号：S816[农业科学—饲料科学] S852.2[农业科学—畜牧学]