离子交换-反相色谱纯化育亨宾  

Purification of crude yohimbine by ion-exchange and reversed-phase chromatography

作　　者：陶蕾[1] 赵明珠[1] 赵凤生[1] 徐伟[2] 王芝祥[2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海医药工业研究院,上海200040

出　　处：《中国生化药物杂志》2011年第5期349-351,355,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：上海市科学技术委员会中药现代化项目(08DZ1973800)资助

摘　　要：目的从育亨宾树皮中提取的育亨宾粗品,一般纯度较低(10%左右)。本文研究纯化育亨宾粗品的方法。方法结合离子交换与反相色谱两种方法,考察了洗脱条件对纯化效果的影响。结果采用1×2型阳离子交换树脂,以乙醇-水-氨水(40∶55∶5)为洗脱液,洗脱的育亨宾纯度最高;然后用C18反相色谱进一步纯化,流动相为甲醇-水(含0.01%三氟乙酸),甲醇浓度按3%,30%,40%,95%分阶段增加,洗脱液中的育亨宾纯度提高至71.2%。结论这项工艺具有成本低、收率高、纯度好、步骤少的特点,在天然产物的分离纯化方面具有潜在的应用价值。Purpose Crude yohimbine separated from Corynanthe yohimbe is only with low purity（about 10%）.A method to purify crude yohimbine should be studied.Methods Ion-exchange and reversed-phase chromatography were combined in this study.The influence of elution conditions on purifying effects was investigated.Results By adsorption with 1×2 cation exchange resin and elution with mixture of ethanol,water and ammonia（40∶55∶5）,highest purity of yohimbine was obtained.Next separation with C18 reversed-phase chromatography was eluted with the mixture of methanol and water（containing 0.01% trifluoroacetic acid）.When the concentration of methanol increased progressively based on 3%-30%-40%-95%,the purity of yohimbine in eluent could increase to 71.2%.Conclusion This technique has advantages of low cost,high yield,good purity and fewer procedures and has potential application value in the separation and purification of natural products.

关 键 词：育亨宾 纯化 离子交换 反相色谱 

分 类 号：TQ460.6[化学工程—制药化工] R284.2[医药卫生—中药学]