纳米银生物海绵的制备及抗菌性能  

Study on the preparation of silver nanoparticles-loaded bio-sponge and its antibacterial efficacy

作　　者：李东红[1] 刁俊林[1] 刘建仓[1] 胥婷[1] 

机构地区：[1]创伤、烧伤、复合伤国家重点实验室,第三军医大学,大坪医院野战外科研究所第二研究室,重庆400042

出　　处：《中国生化药物杂志》2011年第5期352-355,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：军队"十一五"课题(06MA201);创伤;烧伤;复合伤国家重点实验室课题(SKLZZ200812)

摘　　要：目的研究纳米银生物海绵的制备方法及其抑菌能力。方法含有硝酸银的壳聚糖等胶液经复合交联、冷冻干燥,最后经紫外线照射得载纳米银的生物海绵;扫描电镜观测纳米银粒径;扩散法测定其对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的体外抑制作用,并与明胶海绵比较;大鼠后背创面涂抹混合菌液后,贴敷海绵,于不同时间点观测伤口愈合情况,取分泌液进行细菌培养考察海绵的在体抑菌能力。结果扫描电镜结果显示海绵中纳米银的粒径多为40 nm;相对于明胶海绵和生物海绵,载纳米银生物海绵对三个菌种无论是离体或在体都有一定的抑制作用,特别是对铜绿假单胞菌的抑菌作用更为明显。结论纳米银生物海绵对常见病菌有一定的抑制作用,可望作为一种新型的生物敷料用于预防和治疗创面感染。Purpose To study the preparation of silver-nanoparticles-loaded bio-sponge and its antibacterial efficacy.Methods The silver-nanoparticles-loaded bio-sponge was prepared by cross-linking a gum-solution which was composed of silver nitrate,chotisan,algin and collagen protein,and followed by lyophilizing and ultraviolet irradiation.The particle diameter of silver nanoparticle was determined by SEM.The antibacterial performance of the sponge against S.aureus,E.coli,and P.aeruginosa in vitro was measured by diffusion test.After painting a mixed bacterial solution on the trauma of the back,the mice were dressed with gelatin sponge and silver-nanoparticles-loaded sponge respectively,and the exudate of the trauma was collected to determine the category and quantity of the bacteria.Results The result of the SEM indicated that the diameters of most particles were 40 nm.Comparied with gelatin sponge and bio-sponge,silver-nanoparticle-loaded bio-sponge displayed obvious antibacterial efficacy,especially on the P.aeruginosa,regardless of in vitro or in vivo. Conclusion It is anticipated that silver-nanoparticle-loaded bio-sponge can be used as a novel bio-dressing to prevent and treat the infected trauma.

关 键 词：生物海绵 纳米银 制备 抑菌性能 

分 类 号：R944.9[医药卫生—药剂学] R961[医药卫生—药学]