杂交鳢及其亲本同工酶的比较  

Comparative study on isozyme of Ophiocephalus argus Cantor (♂), Ophiocephalus maculatus(♀) and their hybrids

作　　者：刘凯[1] 谢楠[1] 姚桂桂[1] 刘新轶[1] 王宇希[1] 

机构地区：[1]杭州市农业科学研究院水产研究所,浙江杭州310024

出　　处：《水产养殖》2011年第10期14-17,共4页Journal of Aquaculture

基　　金：杭州市科技重大创新项目(20083212A04)

摘　　要：采用聚丙烯酰胺凝胶垂直板电泳,对浙江乌鳢、广东斑鳢、浙江乌鳢(♂)×广东斑鳢(♀)杂交种3个群体,肝、肌肉、脑、脾、鳃、肾、心7种组织的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)2种同工酶进行了分析。结果表明:2种同工酶在各自群体内具有明显的组织特异性,在浙江乌鳢、广东斑鳢间表现出一定的差异性,其杂交后代与二者有一定的相似性,但同工酶酶谱中也出现了"互补带"和"杂交带",符合杂交育种的一般规律。By means of the polyacrylamidc gradient gel electrophoresis, two isozymes （ MDH and LDH ） from seven tissues （liver, muscle,brain, spleen, gills, kidney and heart）of Ophiocephalus argus Cantor （ ♂ ） , Ophiocephalus maculates （ ♀ ） and their hybrids were studied. The results showed that two isozymes presented obvious tissue. The expression of isozymes showed there was a certain extent discrepancy between Ophiocephalus argus Cantor （ ♂ ） and Ophiocephalus maculates （ ♀ ）,and their hybrids showed similarity with both of them,but ＂hybrid band＂ and ＂complementary band＂ alse were found. The conclusion was in accord with general discipline of cross breeding.

关 键 词：杂交鳢 杂交优势 同工酶 乳酸脱氢酶 苹果酸脱氢酶 

分 类 号：S917.4[农业科学—水产科学]