二种方法检测耐亚胺培南鲍曼不动杆菌产金属β-内酰胺酶的效果比较  

Two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii:A comparative study

作　　者：雷红[1] 董梅[1] 孙敏霞[1] 孟祥红[1] 杨彩娥[1] 赵利[1] 朱蕾[1] 杨昌梅[1] 

机构地区：[1]解放军第309医院检验科,北京100091

出　　处：《军医进修学院学报》2011年第10期990-991,994,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的比较两种方法检测耐亚胺培南鲍曼不动杆菌产金属β-内酰胺酶的效果。方法采用改良Hodge试验和EtestMBL方法检测细菌产金属β-内酰胺酶;采用MicroScan WalkAway 96检测鲍曼不动杆菌的耐药性。结果采用两种方法同时检测40株耐亚胺培南鲍曼不动杆菌的金属β-内酰胺酶,改良Hodge试验阳性36株,阳性检出率90%;Etest MBL方法检测阳性38株,阳性检出率95%。结论两种方法均能简便、快速、准确的检测鲍曼不动杆菌产金属β-内酰胺酶。Objective To compare the two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.Methods Metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii was detected by modified Hodge test and Etest MBL test.Resistance of Acinetobacter Baumanni to imipenem was assayed with McrioScan WalkAway 96.Results The metallo-β-lactamase was detected in 40 imipenem-resistant Acinetobacter Baumannii strains with the two methods.The metallo-β-lactamase was detected in 36 out of the 40 imipenem-resistant Acinetobacter Baumannii strains by modified Hodge test and in 38 imipenem-resistant Acinetobacter Baumannii strains by Etest MBL test,with a positive detection rate of 90% and 95%,respectively.Conclusion The two detection methods are simple,rapid and accurate for detecting the metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.

关 键 词：微生物敏感试验 改良HODGE试验 Etest金属酶试验 

分 类 号：R446.54[医药卫生—诊断学]