胰淀素在肥胖相关性肾病进展中的作用  

The Role of Amylin in obesity-related glomerulopathy

作　　者：刘声茂[1] 崔英春[1] 田向辉[1] 顾华[1] 吴曼[1] 刘庆鑫[2] 张冬梅[1] 

机构地区：[1]吉林大学第二医院肾病内科,吉林长春130041 [2]吉林大学中日联谊医院肾病科

出　　处：《中国实验诊断学》2011年第9期1433-1436,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划项目(200705207)

摘　　要：目的探讨胰淀素(amylin)在肥胖相关性肾病(ORG)发展过程中的作用。方法 14例经肾活检证实的ORG患者为观察组(ORG组),选择同期经肾活检确诊为系膜增生性肾小球肾炎患者作为相关疾病对照组(NC)。20只Wis-tar大鼠,高脂饮食诱导ORG大鼠模型,成模后随机均分为模型对照组(NC)和Amylin组(Amylin)。采用ELISA法检测血清amylin;放射免疫法检测胰岛素水平;自动生化分析仪测定血糖、血脂;考马斯亮蓝方法检测24小时尿蛋白定量;免疫组化法检测肾小球内amylin的表达。结果 ORG患者血清内amylin水平明显高于系膜增生性肾小球肾炎患者,且肾脏有amylin沉积;Amylin皮下注射组大鼠血清amylin、胰岛素、血脂水平均较ORG组升高,24小时尿蛋白定量增加。结论 Amylin参与ORG的进展,促进病情进展。Objective In order to explore the role of amylin in the progess of obesity related glomerulopathy.Methods 14 biopsy-proved ORG patients in observed group and 14 biopsy-proved mesangial proliferative glomerulonephritis patients in control group were studied.20 Wistar rats were induced by high fat diet to establish obesity related glomerulopathy model,and randomized into 2 equal groups with 10 rats in each group：model control group and Amylin group.Serum Amylin level was determined by ELISA.The serum insulin were detected by radioimmunoassay.The serum glucose and lipid was determined with automatic biochemical analyzer,The 24-hour urine protein was detected with Coomassie brilliant blue method.Amylin expressed in kidney of rat by immunochemistry.Results The levels of serum Amylin in patients of group ORG was high than in group NC.As well as the deposition of amylin in kidney was obvious.The levels of Amylin,insulin and lipid in serum,and proteinuria in group Amylin were high than in group ORG.Conclusion Amylin takes part in the occurrence and development of ORG,and has played apositive role.

关 键 词：胰淀素肥胖相关性肾病 胰岛素抵抗 

分 类 号：R589.2[医药卫生—内分泌] R692[医药卫生—内科学]