大鼠脑为底物间接免疫荧光法检测谷氨酸脱羧酶抗体的研究  

Study on GAD Antibodies with indirect immuno-fluorescence by using rat's cerebral tissue as substrate

作　　者：陈志英[1] 白梅[1] 孟和[1] 其其格[1] 

机构地区：[1]解放军第253医院,内蒙古呼和浩特010051

出　　处：《中国实验诊断学》2011年第9期1436-1437,共2页Chinese Journal of Laboratory Diagnosis

基　　金：内蒙古自治区自然科学基金(990302-5)

摘　　要：目的寻找一种更简便快速的谷氨酸脱羧酶抗体(GADab)的检测方法。方法制备小鼠、大鼠脑印片、人胰腺匀浆涂片,摸索实验条件,建立改良的免疫荧光方法,并与ELISA方法比对,对其特异性、可行性、准确性等进行评估。结果所建立的方法与现行的酶免疫吸附法和以人胰腺为底物的传统免疫荧光法对GADab的检测结果无显著差异。结论大鼠脑可代替正常人胰腺用于GADab免疫荧光检测。Objective To look out a kind of rapid and simple mothod for GADab detection：the new indirect immuno-fluorescence.Methods to make substrates with mice＇s brain or rat＇s cerebral tissue.Results There were no significant significant difference between brain of mice and rat＇s from normal human＇s pancreas.in testing GADab with indirect immuno-fluorescence technique or ELISA.Conclusion it is suggested that rat＇s cerebral tissue could be for normal human＇s pancreas in immuno-fluorescence for testing GADab.

关 键 词：GAD抗体 间接免疫荧光法 鼠脑 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]