糖尿病大鼠蛋白尿与肾脏病理及nephrin表达的相关性研究  

Association between albuminuria and expression of nephrin in diabetic rats

作　　者：屈智慧[1] 杨立志[1] 赵颖[1] 肖庆飞[1] 崔明姬[1] 

机构地区：[1]吉林大学第一医院肾内科,吉林长春130021

出　　处：《中国实验诊断学》2011年第9期1441-1444,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨糖尿病大鼠蛋白尿的排泄量与大鼠肾脏病理改变及足细胞裂孔蛋白nephrin表达的相关性。方法将40只Wistar大鼠分为正常组(NCn=20)、糖尿病组(DMn=20),DM组单次腹腔注射链脲菌素(STZ)50 mg/kg,建立糖尿病模型,NC组注射等量枸橼酸缓冲液。分别于0周、4周8、周、12周动态监测大鼠尿白蛋白排泄量、肾重/体重指数(KW/BW),肌酐清除率(Ccr)等。取不同时期糖尿病大鼠肾组织分别行常规病理检查和用免疫组化检测大鼠肾脏nephrin的表达。结果与NC组相比,DM组大鼠随着糖尿病病程的延长尿白蛋白、肌酐清除率逐渐增加,肾重/体重指数增高,并且白蛋白尿早于肾功能的改变,光镜显示DM组大鼠肾小球细胞外基质增多,基底膜增厚,系膜基质增多。随着病理改变加重,肾组织内nephrin蛋白表达开始降低,在12周时最明显。结论糖尿病大鼠出现白蛋白尿,是肾脏损伤的早期指标,随着尿蛋白的增多,肾脏病理改变加重且nephrin蛋白表达降低,提示nephrin蛋白参与糖尿病大鼠蛋白尿的产生与发展。Objective To observe albuminuria excretion in diabetic rats and to investigate its relationships with renal tissue pathological changes and the expression of nephrin.Methods 40 Wistar rats were divided into control group（NC n=20）,diabetic group（DM n=20）.Diabetes was induced by peritoneal injection of STZ 50 mg/kg.Urinary albumin（UA）,renal fuction and the ratio of kidney weight/body weight（KW/BW） were detected dynamically at 0,4,8,12 weeks.The renal tissue were analyzed by by light microscope.Expression of nephrin was detected by immunohistochemistry.Results Compared with NC,with extension of the course of disease,urinary albumin,Ccr and the ratio of kidney weight/body weight in DM were gradually increased（P〈0.05）.Albuminuria appeared earlier than abnormalities of Ccr.Histological examination of the kidney revealed increased ECM accumulation,thickening of the glomerular basement membrane with the development of diabetic nephropathy.Nephrin was distributed asymmetry and decreased gradually on glomeruloes in diabetic group especially at 12 th week.Conclusion Albuminuria appeared earlier in diabetic rats,which is an indicator of early damage in diabetic nephropathy.Nephrin may play a role in pathogenesis and development of the Albuminuria in diabetic nephropathy.

关 键 词：糖尿病大鼠 尿白蛋白 NEPHRIN 

分 类 号：R587.1[医药卫生—内分泌]