组分对含羟基磷灰石/脂肪族聚氨酯复合骨组织工程支架的理化性能及生物学性能的影响  

Effect of Composition on Physical-chemical Properties and Biological Properties of Hydroxyapatite/Aliphatic Polyurethane Scaffolds for Bone Tissue Engineering

作　　者：王立[1] 左奕[1] 邹琴[1] 李玉宝[1] 

机构地区：[1]四川大学纳米生物材料研究中心,分析测试中心,成都610064

出　　处：《高等学校化学学报》2011年第10期2453-2459,共7页Chemical Journal of Chinese Universities

基　　金：国家“九七三”计划项目(批准号:2007CB936102);国际科技合作和与交流专项(中荷战略联盟项目)(批准号:2008DFB50120);国家自然科学基金(批准号:50972096)资助

摘　　要：以异佛尔酮二异氰酸酯为聚氨酯硬段,通过原位聚合使聚合过程中释放的气体发泡,制备了用作骨组织工程材料的羟基磷灰石/脂肪族聚氨酯多孔支架.系统考察了不同组成配方,即羟基磷灰石(HA)含量、发泡剂含量以及聚氨酯(PU)软硬段的比例对三维支架材料的机械性能和微观孔隙结构等的影响,并通过体外细胞培养和体内肌肉植入初步评价了该复合支架材料的生物学性能.结果显示,HA和PU硬段含量的增加能有效增强支架的力学强度;发泡剂(水)含量的改变影响了多孔支架的孔隙结构,水含量越高,平均孔径越大;支架的孔隙率随HA及PU硬段含量增加而增大,物料黏度的增加使得多孔体系在发泡产生气体时不易垮塌.MG63成骨表型细胞在三维支架上可以很好地黏附铺展、生长和增殖,形成了骨细胞特有的多层融合,表明复合材料有很好的细胞亲和性,不同比例的材料对细胞增殖影响的差异不大.动物实验结果显示,复合支架材料的生物学反应温和,具有良好的生物相容性.HA/PU三维多孔支架将在骨组织工程领域有较好的应用前景.Aliphatic polyurethane（PU） and hydroxyapatite（HA） composite scaffolds were prepared by in-situ polymerization and gas self-foaming process.The morphologies and properties of the scaffolds were studied by varying the contents of HA,foaming agent（water） and soft/hard segment ratio and characterized by scanning electron microscope（SEM）,X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FTIR） and mechanical test.The results show that the compressive strength of the scaffolds increased with both the HA contents and the hard segment contents.The scaffold porous structure was affected by HA content and foaming agent.The scaffold porosity increased with HA contents and decreased with the hard segment contents.The average pore size of the scaffold also was increased with the contents of foaming agent.MTT results and SEM observation of cells showed that the porous structure of HA/PU scaffold provides a good microenvironment for cell adherence,growth and proliferation.After implantation in rat muscle,the scaffold was well tolerated by host tissues.The porous HA/PU scaffold provides a good perspective for designable tissue-engineering scaffolds.

关 键 词：骨组织工程支架 羟基磷灰石 脂肪族聚氨酯 组分影响 生物学性能 

分 类 号：O631[理学—高分子化学]