甘油对丝素蛋白膜水溶性和结构的影响  

Relationship Between Structure and Solubility of Silk Fibroin and Glycerol in Blend Film

作　　者：罗敏清 卢神州[1,2] 

机构地区：[1]现代丝绸国家工程实验室 [2]苏州大学纺织与服装工程学院,苏州215123

出　　处：《高等学校化学学报》2011年第10期2460-2464,共5页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目(批准号:2005CB623906);江苏省产学研联合创新资金项目(批准号:BY2009127)资助

摘　　要：探讨了甘油的加入对丝素蛋白溶解过程的结晶结构及结晶度的影响.以甘油为添加剂用流延法于室温制备一系列丝素共混膜,测试了其含水率、溶失率及结构和机械性能.结果表明,随着甘油加入量的增加,丝素蛋白的溶失率逐渐降低;当甘油/丝素质量分数超过10%时,共混膜呈现水不溶性.当甘油加入量较少时,丝素蛋白呈现少量的SilkⅡ结晶,而SilkⅠ结构不明显.随着甘油含量的不断增加,SilkⅠ结晶逐渐增加而SilkⅡ结晶逐渐减少.当甘油加入量达到不溶点(10%)时,丝素蛋白主要转变为SilkⅠ结晶,而几乎没有SilkⅡ结晶.甘油的加入可使共混膜的柔韧性显著提高,并促使丝素蛋白结晶度提高以及促使丝素蛋白向SilkⅠ结晶转变,从而降低丝素蛋白膜的水溶性.The change of crystal structure and crystallinity of silk fibroin（SF） in the process of becoming insoluble by adding glycerol was studied.The blend silk fibroin films were casted by silk fibroin solutions mixing with glycerol at a series of ratios at room temperature.The water-content,solubility,structure,and mechanical properties of the blend films were measured.The results show that with the content of glycerol increasing,the solubility decreases.The blend films became insoluble when the mass ratios of glycerol/SF are more than 10%.When the content of glycerol is low,there is a little of Silk Ⅱ structure and almost no Silk Ⅰ structure in blend film.With glycerol increasing,the crystallinity of Silk Ⅰ increases while that of Silk Ⅱ decrease in blend film.When the content of glycerol exceeding 10%,in which content that silk fibroin become insoluble,the crystalline structure of SF is mostly changed into Silk Ⅰ and there is almost no Silk Ⅱ structure.Mechanical properties indicated that glycerol could significantly improved the flexibility of silk films.In summary,the crystal structure is changed to Silk Ⅰ and the crystallinity of silk fibroin is increased so that the solubility of silk fibroin is significantly reduced by adding glycerol when casting film.

关 键 词：甘油 丝素蛋白 膜 结晶结构 结晶度 机械性能 

分 类 号：O631[理学—高分子化学]