HPLC测定脐带血HbF正常参考值的建立及对母血污染的评价分析  

Establishment of the reference range for normal values of HbF in cord blood and analysis for maternal blood contamination of cord blood

作　　者：汤丽霞[1] 欧丽群[1] 曾劲伟[1] 梁彩红[1] 禤洁甜[1] 林蔚[1] 陈华锦[1] 杨光[1] 

机构地区：[1]广东省佛山市第一人民医院临床研究所,528000

出　　处：《重庆医学》2011年第28期2820-2821,共2页Chongqing medicine

摘　　要：目的建立高效液相色谱法(HPLC)检测妊娠中晚期脐带血胎儿血红蛋白(HbF)的正常参考值范围,通过检测脐带血HbF的浓度筛查母血污染脐带血。方法对329例脐血进行G显带染色体核型分析,应用抗碱法检测HbF 114例,HPLC检测HbF 215例。应用百分位数法界定两种方法测定HbF单侧下限99%可信区间的正常参考值。结果在329例胎儿脐带血染色体核型分析中,有17例染色体多态,17例染色体数目异常,7例染色体结构异常。HPLC检测脐带血HbF单侧下限99%可信区间参考值为79.4%,其中男性胎儿为83.5%,女性胎儿为76.2%。秩和检验男女间HbF平均值差异无统计学意义。结论 HPLC脐带血HbF浓度在脐血染色体检查中可作为一项母血污染脐血的筛查指标。Objective To establish the normal reference values of HbF by HPLC in fetus cord blood on second and third trimester of pregnancy in order to detect maternal blood contamination of cord blood.Methods Analysis of G-banded chromosomes of 329 samples of cord blood was developed.HbF in cord blood of 215 samples detected by HPLC and that of 114 samples by alkali resistant assay.The reference values of 99% confidence interval of HbF determined with the method of percentiles.Results There were 17 cases of chromosome polymorphism,17 cases of numerical abnormalities and 7 cases of structural abnormality of chromosomes among 329 cases of cord blood.The unilateral lower limit of HbF on 99% confidence interval was 79.4% of 215 cases by HPLC,83.5% male and 76.2% female among them,and was 48.7% of 114 cases by alkali-resistant assay,51.7% male and 48.8% female among them.The mean value of HbF between male and female has no significant difference by rank test.Conclusion Concentration of HbF in cord blood can be utilized for screening maternal blood contamination of cord blood.

关 键 词：胎儿血红蛋白 胎血 产前诊断 色谱法 高压液相 

分 类 号：R714.5[医药卫生—妇产科学]