未成熟与成熟猪囊尾蚴蛋白双向电泳的图谱分析  

Total Protein Analysis of Immature and Mature Cysticerci of Taenia solium by Two-dimensional Electrophoresis

作　　者：方文[1] 肖靓靓[2] 包怀恩[2] 牟荣[2] 

机构地区：[1]贵阳医学院医学检验系临床生化教研室,贵州贵阳550004 [2]贵阳医学院人体寄生虫学教研室,贵州贵阳550004

出　　处：《贵州农业科学》2011年第9期134-136,共3页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"三种人体带绦虫入侵中间宿主家猪的分子机制研究"(30660168)

摘　　要：为从蛋白质水平揭示猪带绦虫入侵中间宿主家猪的免疫逃避机制,应用双向电泳技术分析未成熟与成熟猪囊尾蚴蛋白质表达差异,进行双向电泳图像软件分析。结果表明:未成熟猪囊尾蚴与成熟猪囊尾蚴蛋白的双向电泳凝胶上分别有(217±13)个、(241±17)个蛋白斑点,未成熟猪囊尾蚴表达上调2倍以上的蛋白有6个,下调2倍以上的蛋白有2个。In order to explore the immune-escape mechanisms of the T.solium cysticercus on the protein level,the differentially expressed proteins of the immature and mature T.solium cysticerci were separated by two-dimensional electrophoresis respectively and analyzed by ImageMaster 2D Plantinum 6.0 software.The results showed that the immature and mature T.solium cysticerci have 217 13 and 241 17 protein spots respectively,the immature T.solium cysticerci have six proteins of more than two times upregulation and two proteins of more than two times downregulation compared to the mature T.solium cysticerci.

关 键 词：猪带绦虫 囊尾蚴 双向电泳 蛋白质组 

分 类 号：S858.28[农业科学—临床兽医学]