秃鹫肾脏的组织结构及相关活性物质的表达  

Histological structure and expression of inter related polypeptides in Aegypius monachus kidney

作　　者：俞诗源[1] 王锦锦[1] 孙隽[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2011年第5期90-95,共6页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30370163);兰州市发展计划资助项目(2010-03-127)

摘　　要：为搞清秃鹫(Aegypius monachus)肾脏的组织结构特征,观察Bax蛋白、c-Fos蛋白、TGF-β1在肾脏中的表达情况,应用生物显微技术观察了秃鹫肾脏的组织结构,利用免疫组织化学方法检测了Bax蛋白、c-Fos蛋白、TGF-β1在肾脏中的表达.结果表明,秃鹫肾实质由许多肾单位、集合管和少量结缔组织组成;肾单位是肾脏功能结构的基本单位,它由一个肾小体和一个与其连接的上皮性肾小管构成;血管球由一团迂回盘曲的毛细血管丛构成,结构简单;近端小管由单层立方上皮组成,上皮细胞游离面有刷状缘,细胞界限不明显;肾小体血管极有明显的肾小球旁复合体.近端小管上皮细胞呈Bax蛋白、c-Fos蛋白、TGF-β1免疫反应阳性.Bax蛋白、c-Fos蛋白和TGF-β1可能发挥着不同的功能,对肾单位的构筑、肾小管结构的稳定等可能有重要的调节作用.Histological structure is observed by light microscopy,and expression of Bax protein,c-Fos protein TGF-β1 is examined by immunohistochemical methods in Aegypius monachus kidney.Results show that the kidney of Aegypius monachus consists of many nephrons,collecting ducts and a little connective tissue.The nephron is the structural and functional unit of the kidney,comprising renal corpuscle and an epithelial renal tubule.The renal glomerulus is made from convoluted capillaries and its structure is simple.The proximal tubule is lined with simple cuboidal epithelium,with a well-developed brush border（microvilli）.The juxtaglomerular complexs are located in the vascular pole of the renal corpuscles.Bax protein,c-Fos protein,TGF-β1 appeare to be expressed in the epithelial cells of proximal tubules.Bax protein,c-Fos protein,TGF-β1 have different functions.They are very important for formation of nephrons,structurally stable renal tubules in Aegypius monachus kidney.

关 键 词：秃鹫 肾 组织结构 BAX蛋白 C-FOS蛋白 TGF-Β1 免疫组织化学 

分 类 号：Q954.6[生物学—动物学]