农杆菌介导海岛棉胚性愈伤高效遗传转化体系的研究  

Research on High Efficient Genetic Transformation System of Agrobacterium Gossypium barbadense L.Embryogenic Calli

作　　者：周丽容[1,2] 陈全家[1,2] 贺雅婷[1,2] 克尤木[1,2] 李杨阳[1,2] 李琼[1,2] 杨婷[1,2] 袁理星[2] 曲延英[1,2] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学农业生物技术重点实验室,乌鲁木齐830052

出　　处：《新疆农业大学学报》2011年第4期317-320,共4页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(3070051330960201)

摘　　要：以新海30和新海16的胚性愈伤组织为转化受体,用农杆菌介导法将苏云金芽孢杆菌杀虫晶体蛋白(Bt)基因导入新海30和新海16。结果表明,在相同的转化条件下,新海30遗传转化效率大于新海16。培养基中无机盐浓度为1/2MS,凝固剂gelrite浓度为2.0 g/L时,能够提高胚状体萌发和成苗率,降低畸形苗频率。通过T1代PCR及T2代RT-PCR检测证实Bt基因已整合到了海岛棉基因组中,获得转基因植株。This study was conducted to transform employ cullus of Xinhai 30 and Xinhai 16 into a recipient.The Two cultivars of embryogenic calli were transformed into Bacillus thuringiensis toxin protein gene（BT） by Agrobacterium.The results shows that the transformation rate of Xinhai 30 was higher than that of Xinhai 16 under the same transformation condition.The concentration in inorganic salt of lowering substratum was 1/2 MS,the concentration of the coagulator gelrite was 2.0 g/L,which ean improve rate of embryoid germination and reduce rate of deformed seedling.Therefore,Bt gene has been transformed into genomics of Gossypium barbadense L.by detection of T1-PCR and T2-PCR.The transgenic plants were obtained.This study set the base for on a large scale developing the transgenic work of Gossypium barbadense L.

关 键 词：海岛棉 农杆菌 遗传转化 再生植株 转化效率 

分 类 号：S562.035.3[农业科学—作物学]