Box－Behnken效应面法优化石杉碱甲纳米结构脂质载体处方  

Formulation optimization of huperzine A loaded nanostructured lipid carriers by Box-Behnken experimental design

作　　者：杨春荣[1,2] 孙忻[1] 赵秀峰[3] 胡海洋[2] 乔明曦[2] 陈大为[2] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]牡丹江医学院附属医院肿瘤科,黑龙江牡丹江157011

出　　处：《沈阳药科大学学报》2011年第10期769-774,共6页Journal of Shenyang Pharmaceutical University

基　　金：佳木斯大学面上资助项目(L2009-107)

摘　　要：目的采用Box-Behnken效应面法筛选石杉碱甲纳米结构脂质载体最佳处方。方法采用熔融超声-高压匀质法制备石杉碱甲纳米结构脂质载体,分别以混合脂质(X1)、混合乳化剂(X2)和脂药比(X3)为考察对象,以粒径(Y1)、包封率(Y2)和载药量(Y3)为评价指标,利用三因素三水平Box-Behnken效应面设计法筛选石杉碱甲纳米结构脂质载体的最佳处方。结果按最优处方制备的纳米粒粒径为(121.67±3.21)nm、包封率为(89.18±0.28)%、载药量为(1.46±0.05)%,与预测值偏差均小于5%。结论采用Box-Behnken效应面法优化石杉碱甲纳米结构脂质载体处方是有效、可行的。Objective To optimize the formulation of huperzine A loaded nanostructured lipid carriers by Box-Behnken design. Methods Huperzine A loaded nanostructured lipid carriers were prepared by melt ultrasonication-high pressure homogenization method. A three factor, three-level Box-Behnken design was employed, with the amount of the mixed lipid （ X1 ）, emulsifier mixture concentration （ X2 ） and lipid/drug ratio （ X3 ） as the independent variables. The dependent variables were the particle size （ Y1 ）, entrapment efficiency （ Y2 ） and drug loading （ Y3 ）. Results In the optimized formulation, the particle size, entrapment efficiency and drug loading were （ 121.67 ± 3.21 ） nm, （ 89. 18 ±0.28 ） % and （ 1.46 ± 0. 05 ） %, respectively. Moreover, the deviations of the measured values from the predicted ones were less than 5 %. Conclusions The Box- Behnken design is effective and suitable for optimizing the formulation of huperzine A loaded nanostructured lipid carriers.

关 键 词：石杉碱甲 纳米结构脂质载体 熔融超声-高压匀质法 Box—Behnken效应面法 

分 类 号：R94[医药卫生—药剂学]