高效毛细管电泳法测定清胃黄连丸中盐酸小檗碱含量  

Quantitative Determination of Berberine in Qingwei Huanglian Pills by HPCE

作　　者：黄玉芝[1] 房娟娟[1] 李金[1] 闫滨[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中国药业》2011年第19期20-21,共2页China Pharmaceuticals

摘　　要：目的建立测定清胃黄连丸中盐酸小檗碱含量的高效毛细管电泳法。方法毛细管区带电泳法,采用熔融石英毛细管柱(47cm×75μm),有效长度40 cm,缓冲溶液为pH=3.0的60 mmol/L磷酸盐溶液-甲醇(65∶35),分离电压30kV,进样时间5s,毛细管柱温25℃,紫外检测波长200 nm。结果盐酸小檗碱进样质量浓度在0.05～0.45 g/L范围内与峰面积线性关系良好(r=0.999 7),平均加样回收率为100.31%,RSD为2.53%(n=5)。结论该方法测定清胃黄连丸中盐酸小檗碱的含量灵敏、快速、结果可靠。Objective To develop a high performance capillary electrophoresis（HPCE） method for the quantitative determination of berberine in Qingwei Huanglian Pills.Methods The capillary zone electrophoresis was adopted.The uncoated fused silica capillary（47 cm×75 μm,valid length 40 cm） was used to separate the components.The running buffer contained 60 mmol/L sodium dihydrogen phosphate（pH=3.0）-methanol（65 ∶35） under the voltage of 30 kV at 25 ℃and the injection time of 5 s.UV detector was used to monitor at 200 nm.The running time lasted for 20 min.Results The linear calibration range was 0.05-0.45 g/L for berberine.The average recovery rate was 100.14%,RSD 2.52%（n=5）.Conclusion The quantitative determination of berberine in Qingwei Huanglian Pills by this method is sensitive,rapid and reliable.

关 键 词：高效毛细管电泳法 盐酸小檗碱 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]